DIURETIC AND LAXATIVE ACTIVITIES OF MORINGA OLEIFERA SEEDS AND PODS IN MICE

Md. Nazmul  Islam; Md. Kawsar  Hossen; Jagadish Chandra  Joardar; Bishwajit  Bokshi; Asish Kumar  Das; Samir Kumar  Sadhu; Nripendra Nath  Biswas

doi:10.53808/KUS.2020.17.1and2.2006-L

Authors

Md. Nazmul Islam Pharmacy Discipline, Life Science School, Khulna University, Bangladesh
Md. Kawsar Hossen Pharmacy Discipline, Life Science School, Khulna University, Bangladesh
Jagadish Chandra Joardar Soil, Water and Environment Discipline, Life Science School, Khulna University, Bangladesh
Bishwajit Bokshi Pharmacy Discipline, Life Science School, Khulna University, Bangladesh
Asish Kumar Das Pharmacy Discipline, Life Science School, Khulna University, Bangladesh
Samir Kumar Sadhu Pharmacy Discipline, Life Science School, Khulna University, Bangladesh
Nripendra Nath Biswas Pharmacy Discipline, Life Science School, Khulna University, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2020.17.1and2.2006-L

Keywords:

Moringa oleifera, Pods, Seeds, Laxative, Diuretic, Electrolytes, Acute Toxicity

Abstract

Moringa oleifera (Family- Moringaceae) is a well-known vegetable for its health benefits for thousands of years having diverse nutritional bioactive constituents. Phytochemical analysis of ethanolic extract of this plant indicated the presence of carbohydrate, reducing sugar, combined reducing sugar, glycosides, tannins (absent in seeds), alkaloids, flavonoids, saponins and steroids. In diuretic activity test, the extract showed a significant (p<0.001) effect at the dose of 200 & 400 mg/kg body weight in production of total urine volume in comparison with standard drug furosemide (5 mg/kg). Upon electrolyte analysis of excreted urine, the extract was found to increase Na+, K+, Clexcretion and may act as loop diuretics. The pods and seeds extract showed significant (p<0.01) laxative effects in mice in terms of stool production with soft consistency at the dose of 200 & 400 mg/kg in comparison with standard drug bisacodyl (10 mg/kg). The most edible part of this plant is pods which showed better diuretic and laxative effects than seeds. So, the pods may be a good source of bioactive constituents which may be used as parent compound(s) for discovering better diuretic or laxative drugs.

Downloads

Download data is not yet available.

References

Amuthan, A., Chogtu, B., Bairy, K., Prakash, M. (2012). Evaluation of diuretic activity of Amaranthus spinosus Linn. aqueous extract in Wistar rats. Journal of Ethnopharmacology, 140(2), 424-427.

Arezoomandan, R., Kazerani, H.R., Behnam-Rasooli, M. (2011). The laxative and prokinetic effects of Rosa damascena Mill in rats. Iranian Journal of Basic Medical Sciences, 14(1), 9-16.

Bhavin, V., Ruchi, V., & Dd, S. (2011). Diuretic Potential of Whole Plant Extracts of Pergularia daemia (Forsk.). Iranian Journal of Pharmaceutical Research, 10(4), 795–798.

Caceres, A., Saravia, A., Rizzo, S., Zabala, L., Leon, E.D., Nave, F. (1992). Pharmacologic properties of Moringa oleifera: 2: Screening for antispasmodic, anti-inflammatory and diuretic activity. Journal of Ethnopharmacology, 36, 233–237.

Das, AK., Ahmed, F, Biswas, NN, Dev, S, & Masud, MM. (2005). Diuretic activity of Acalypha indica. Dhaka University Journal of Pharmaceutical Sciences, 4(1).

Gadge, N.B., Jalalpure, S.S. (2011). Natriuretic and saluretic effects of Hemidesmus indicus R. Br. root extracts in rats. Indian Journal of Pharmacology, 43(6), 714.

Gadge K.R.V. (2006). Anti-Arthritic Activity of extracts of Moringa oleifera lam. Roots, Indian drugs, Scientific and Research Publication from Indian drugs manufacturer’s Association, 43(7):543-6.

Fernandes, Evangeline E, Pulwale, Anubha V, Patil, Gauri A, & Moghe, Alpana S. (2016). Probing regenerative potential of Moringa oleifera aqueous extracts using in vitro cellular assays. Pharmacognosy Research, 8(4), 231.

Friis, C. (1983). Renal excretion of drugs during postnatal development in piglets. Veterinary Research Communications, 7(1), 349-352.

Golla, U., Gajam, P.K., Bhimathati, S.S. (2014). Evaluation of diuretic and laxative activity of hydroalcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 12(4), 372-378.

Harris, Lucinda A. (2006). Current mechanisms of action in treatment of chronic constipation and irritable bowel syndrome. Advanced Studies in Medicine, 6(4 A).

Huizinga, J.D., Ambrous, K., Der-Silaphet, T. (1998). Co-operation between neural and myogenic mechanisms in the control of distension-induced peristalsis in the mouse small intestine. Journal of Physiology, 506(3), 843-856.

Jouad, H., Lacaille-Dubois, M., Lyoussi, B., Eddouks, M. (2001). Effects of the flavonoids extracted from Spergularia purpurea Pers. on arterial blood pressure and renal function in normal and hypertensive rats. Journal of Ethnopharmacology, 76(2), 159-163.

Kane, S.R., Apte, V.A., Todkar, S.S., Mohite, S.K. (2009). Diuretic and laxative activity of ethanolic extract and its fractions of Euphorbia thymifolia Linn. International Journal of Chem Tec Research, 1(2), 149-152.

KP, Shivalinge Gowda, C.M, Mahesh, & Kumar, V. (2009). Study on the diuretic activity of Cynodon dactylon root stalk extract in albino rats. Research Journal of Pharmacy and Technology, 2(2), 338-340.

Kumar, H. S., & Jafrin, L. (2016). Diuretic effect of chloroform seed extract of Moringa oleifera (Linn.) in Wistar rats. International Journal of Basic and Clinical Pharmacology, 5(6), 2561-2565.

Martin-Herrera, D., Abdala, S., Benjumea, D., Gutierrez-Luis, J. (2008). Diuretic activity of some Withania aristata Ait. fractions. Journal of Ethnopharmacology, 117(3), 496-499.

Morton, J.F. (1991). The horseradish tree, Moringa pterigosperma (Moringaceae). A boon to arid lands. Economic Botany, 45, 318–333.

Otshudi, A.L., Vercruysse, A., Foriers, A. (2000). Contribution to the ethnobotanical, phytochemical and pharmacological studies of traditionally used medicinal plants in the treatment of dysentery and diarrhoea in Lomela area, Democratic Republic of Congo (DRC). Journalof Ethnopharmacology, 71(3), 411-423.

Patel, B.R., Ashok, B., Ravishankar, B. (2011). Study on the diuretic activity of Veerataru kwatha in albino rats. Ayu, 32(3), 395.

Parrotta, J. A. (1993). Moringa oleifera Lam: Resedá, Horseradish Tree, Moringaceae, Horseradish-tree Family. International Institute of Tropical Forestry, US Department of Agriculture, Forest Service.

Sayana, S.B., Khanwelkar, C.C., Nimmagadda, V.R., Chavan, V.R. (2014). Antiurolithic activity of aqueous extract of roots of Cissampelos pareira in albino rats. Asian Joiurnal of Pharmaceutical and Clinical Research, 7(3), 49-3.

Saito, T., Mizutani, F., Iwanaga, Y., Morikawa, K., Kato, H. (2002). Laxative and anti-diarrheal activity of polycarbophil in mice and rats. Japanese Journal of Pharmacology, 89(2), 133-141.

Shulman, N. B., Ford, C. E., Hall, W. D., Blaufox, M. D., Simon, D., Langford, H. G., & Schneider, K. A. (1989). Prognostic value of serum creatinine and effect of treatment of hypertension on renal function. Results from the hypertension detection and followup program. The hypertension detection and follow-up program cooperative group. Hypertension, 13(5_supplement), I80.

Tirumalasetty, J., Chandrasekhar, N., Naveen, A. (2015). Evaluation of diuretic activity of ethanol extract of Benincasa hispida stem in Swiss albino rats. Journal of Chemical and Pharmaceutical Research, 5 (3), 91–97.

Vilanova-Sanchez, A., Gasior, A.C., Toocheck, N., Weaver, L., Wood, R.J., Reck, C.A., Wagner, A., Hoover, E., Gagnon, R., Jaggers, J. (2018). Are Senna based laxatives safe when used as long term treatment for constipation in children? Journal of Pediatric Surgery, 53(4), 722-727.

Waterman, S.A., Costa, M. (1994). The role of enteric inhibitory motoneurons in peristalsis in the isolated guinea-pig small intestine. Journal of Physiology, 477(3), 459-468.

Wile, D. (2012). Diuretics: a review. Annals of Clinical Biochemistry, 49(5), 419-431.

Yang, Z. H., Yu, H. J., Pan, A., Du, J. Y., Ruan, Y. C., Ko, W. H., Chan H. C., Zhou, W. L. (2008). Cellular mechanisms underlying the laxative effect of flavonol naringenin on rat constipation model. PLOS One, 3(10), e3348.

Zhigila, D.A. (2014). Taximetric study on varieties of Moringa oleifera lam. and Adansonia digitata L.