IN VITRO MORPHOGENESIS IN TWO INDIGENOUS RICE (Oryza sativa L.) CULTIVARS THROUGH DEHUSKED SEED CULTURERE
DOI:
https://doi.org/10.53808/KUS.2017.14.1and2.1618-LKeywords:
Local Aus rice, dehusked seeds, callus induction, in vitro regeneration, survival.Abstract
The local cultivars of rice are the important reservoirs of many valuable traits that need special attention for their conservation and improvement through breeding. To improve the potentiality of using these cultivars into breeding program, standardized protocol is required for their regeneration. The present investigation was carried out to find out the potentiality of two indigenous rice cultivars for callus induction, plant regeneration and to determine the most suitable concentration and combination of growth regulators for the purpose. Mature dehusked seeds of two
local rice cultivars- Chamak and Shama were cultured on MS media supplemented with different concentrations and combinations of 2,4-D(2,4-dichlorophenoxy acetic acid) and NAA( - naphthalene acetic acid) for callus culture. For plant regeneration MS medium fortified with 0.5 mgL1NAA and 3.0mgL-1 BAP was applied. Between these two cultivars, Shama and MS media supplemented with 2,4-D 1.5 mgL-1 and NAA 1.0 mgL-1 showed promising performance in frequency of callus induction (100%), weight of callus (1.90 g), diameter of callus (9.04 mm), color (white and cream) and texture of callus (compact). The cultivar Shama and MS media supplemented with 2,4-D 1.5 mgL-1 and NAA 1.0 mgL-1alsoshowed better performance in plant regeneration (75%caulogenesis, 13.84%calulo-rhizogenesis, 94.99%total morphognesis and 92%root regeneration frequency in root inducing medium)and average survival percentage(45.62%)of the regenerated plants. In this respect, the cultivar Shama and MS media supplemented with 2,4-D 1.5 mgL-1+NAA 1.0 mgL-1 may be recommended for the study of somaclonal variation and for genetic engineering studies.This study will serve as a base line for in vitro somaclonal variation of local rice cultivars.
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