IN VITRO MORPHOGENESIS IN TWO INDIGENOUS RICE (Oryza sativa L.) CULTIVARS THROUGH DEHUSKED SEED CULTURERE
DOI:
https://doi.org/10.53808/KUS.2017.14.1and2.1618-LKeywords:
Local Aus rice, dehusked seeds, callus induction, in vitro regeneration, survival.Abstract
The local cultivars of rice are the important reservoirs of many valuable traits that need special attention for their conservation and improvement through breeding. To improve the potentiality of using these cultivars into breeding program, standardized protocol is required for their regeneration. The present investigation was carried out to find out the potentiality of two indigenous rice cultivars for callus induction, plant regeneration and to determine the most suitable concentration and combination of growth regulators for the purpose. Mature dehusked seeds of two
local rice cultivars- Chamak and Shama were cultured on MS media supplemented with different concentrations and combinations of 2,4-D(2,4-dichlorophenoxy acetic acid) and NAA( - naphthalene acetic acid) for callus culture. For plant regeneration MS medium fortified with 0.5 mgL1NAA and 3.0mgL-1 BAP was applied. Between these two cultivars, Shama and MS media supplemented with 2,4-D 1.5 mgL-1 and NAA 1.0 mgL-1 showed promising performance in frequency of callus induction (100%), weight of callus (1.90 g), diameter of callus (9.04 mm), color (white and cream) and texture of callus (compact). The cultivar Shama and MS media supplemented with 2,4-D 1.5 mgL-1 and NAA 1.0 mgL-1alsoshowed better performance in plant regeneration (75%caulogenesis, 13.84%calulo-rhizogenesis, 94.99%total morphognesis and 92%root regeneration frequency in root inducing medium)and average survival percentage(45.62%)of the regenerated plants. In this respect, the cultivar Shama and MS media supplemented with 2,4-D 1.5 mgL-1+NAA 1.0 mgL-1 may be recommended for the study of somaclonal variation and for genetic engineering studies.This study will serve as a base line for in vitro somaclonal variation of local rice cultivars.
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References
Abe, T., & Futsufura, Y. (1984). Varietal differences of plant regeneration from root derived callus tissue in rice. Japanese Journal of Breeding, 34 (2), 147-155
Abeyaratne, W. M., De Silva, U. N., Kumari, H. M. P. S., & Abeysiriwardena, D. S. De Z. (2004). Callus Induction, Plant Regeneration and Occurrence of Somaclonal Variation in Somatic Tissue of Some Indica Rice Varieties. Annals of the Sri Lanka Department of Agriculture, 6, 1-11
Chawla, H.S. (2004). Introduction to Plant Biotechnology (2nd ed.). Oxford& IBH Publishing Co. Pvt. Ltd.,New Delhi
Chowdhury, G.G.M., Ashrafuzzaman, M., Raihan, F., & Prodhan, S. H. (2012). Comparative Study on Regeneration Potentiality of Aromatic Indica Rice (Oryza sativa L.) of Bangaldesh. Research Journal of Biology, 2(3), 98-103
Chu, Q.R.,&Croughan, T.P.(1990). Genetics of plant regeneration in immature panicle culture of rice. Crop Science, 30, 1194
Ganeshan, S., Baga, M., Harwey, B.L., Rossnagel, B.G., Scoles, G.J., & Chibbar, R.N. (2003). Production of multiple shoots from thiadiazuron-treated mature embryos and leaf-base/apical meristems of barley (Hordeumvulgare L.). Pant Cell Tissue Organ Culture, 73, 57–64
Guo, C.Y.,& Cao, Z.Y. (1982). Effect of different genotypes on induction frequency in anther and scutellum culture of maize in vitro. Hereditess, 4, 8-10
Hoque, E.H.,& Mansfield, J.W. (2004). Effect of genotype and explants age on callus induction and subsequent plant regeneration from root-derived callus of Indica rice genotypes. Plant Cell Tissue and Organ Culture, 78, 217-223
Islam, M.M., Ahmed, M., & Mahaldar, D. (2005). In Vitro Callus Induction and Plant Regeneration in Seed Explants of Rice (Oryzasativa L.). Research Journal of Agriculture and Biological Sciences, 1(1), 72-75
Islam, M.M., Wahed, S.A.,& Khan, S.A.K.U. (2004). Studies on Callus Induction and Regeneration from Dehusked Rice (Oryza sativa L.) Seeds. Plant Tissue Culture, 14(2), 155-160
Kabir, N.,&Basu, S. (2006). Effect of Varieties and Calcium Silicate on Callus Induction and Morphogenesis in Dehusked Rice Seed Culture. B.Sc. Thesis, Khulna University, Khulna, pp. 42.
Khanna, H.K.,& Raina, S.K. (1998). Genotype and culture media interaction effects on regeneration response of three indica rice cultivar. Plant Cell Tissue and Organ Culture, 52, 145
Lai, K.L.,& Liu, L. F. (1981). Induction and plant regeneration of callus from immature embryos of rice plants (Oryza sativa L). Japan Journal of Crop Science, 51 (1), 70-74.
Pandey, S.K., Ramesh, B., & Gupta, P.K. (1994). Study of Effect on Genotype and Culture Medium on Callus Formation and Plant Regeneration in Rice (Oryza sativaL.). Indian Journal of Genetics, 54, 293-299
Rahim, M.A., Hakim, L.,& Miah, A.J. (1991). Induction of callus and plant regeneration. Plant Tissue Culture, 1(1), 27-32
Sikder, M.B.H., Sen, P.K., Mamun, M.A.A., Ali, M.R., & Rahman, S.M. (2006). In Vitro Regeneration of Aromatic Rice (Oryza sativa L.). International Journal of Agriculture &Biology, 4(4), 451-458
Zhang, X., & Te-chato, S. (2012). Callus induction and plantlet regeneration from mature embryos of indica rice (Oryza sativa L.) cultivar Kra Dang Ngah, Journal of Agricultural Technology, 8(7), 2423-2433.
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