OXYMATRINE (AN INHIBITOR OF HSPA8) REDUCES BINDING OF  VGF DERIVED BIOACTIVE PEPTIDE TLQP-21 TO THE SURFACE OF  LIVE SH-SY5Y CELLS

Md Shamim  Akhter; Jesus Rodriguez  Requena

doi:10.53808/KUS.2017.14.1and2.1701-L

Authors

Md Shamim Akhter Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, Bangladesh
Jesus Rodriguez Requena Cimus Biomedical Research Institute, University of Santiago de Compostela –IDIS, Santiago de Compostela 15782, Spain

DOI:

https://doi.org/10.53808/KUS.2017.14.1and2.1701-L

Keywords:

VGF, TLQP-21, SH-SY5Y, OMTR, HSPA8

Abstract

Use of active site-targeting inhibitor is an effective approach leading to pharmacological inventions. Heat shock cognate protein A8 (HSPA8) has been found as a receptor of VGF derived bioactive peptide TLQP-21 in SH-SY5Y cells. So, it was of much interest to carry out this study in order to observe whether Oxymatrine (OMTR), an inhibitor of HSPA8, inhibits the binding of TLQP-21 to the surface of intact, live SH-SY5Y cells. The results confirmed, as expected, that OMTR reduces the binding of TLQP-21 to the surface of intact, live SH-SY5Y cells, with a strong conclusion that the binding of TLQP-21 to the surface of SH-SY5Y cell model was through HSPA8. The inhibition efficacy of OMTR potentiates its application in drug targeting.

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