OXYMATRINE (AN INHIBITOR OF HSPA8) REDUCES BINDING OF VGF DERIVED BIOACTIVE PEPTIDE TLQP-21 TO THE SURFACE OF LIVE SH-SY5Y CELLS

Authors

  • Md Shamim Akhter Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, Bangladesh
  • Jesus Rodriguez Requena Cimus Biomedical Research Institute, University of Santiago de Compostela –IDIS, Santiago de Compostela 15782, Spain

DOI:

https://doi.org/10.53808/KUS.2017.14.1and2.1701-L

Keywords:

VGF, TLQP-21, SH-SY5Y, OMTR, HSPA8

Abstract

Use of active site-targeting inhibitor is an effective approach leading to pharmacological inventions. Heat shock cognate protein A8 (HSPA8) has been found as a receptor of VGF derived bioactive peptide TLQP-21 in SH-SY5Y cells. So, it was of much interest to carry out this study in order to observe whether Oxymatrine (OMTR), an inhibitor of HSPA8, inhibits the binding of TLQP-21 to the surface of intact, live SH-SY5Y cells. The results confirmed, as expected, that OMTR reduces the binding of TLQP-21 to the surface of intact, live SH-SY5Y cells, with a strong conclusion that the binding of TLQP-21 to the surface of SH-SY5Y cell model was through HSPA8. The inhibition efficacy of OMTR potentiates its application in drug targeting.

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Published

29-11-2017

How to Cite

[1]
M. S. . Akhter and J. R. . Requena, “OXYMATRINE (AN INHIBITOR OF HSPA8) REDUCES BINDING OF VGF DERIVED BIOACTIVE PEPTIDE TLQP-21 TO THE SURFACE OF LIVE SH-SY5Y CELLS”, Khulna Univ. Stud., pp. 71–82, Nov. 2017.