PHYTOCHEMICAL AND PHARMACOLOGICAL SCREENING OF Enhydra fluctuans Lour.

Authors

  • Shamsunnahar Khushi Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • Md. Jamil Akhter Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • Md. Mahadhi Hasan Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • Md. Anisur Rahman Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • Gazi Md. Monjur Murshid Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • Samir Kumar Sadhu Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2016.13.1.1501-L

Keywords:

E. fluctuans; antioxidant activity, DPPH, total phenolics, analgesic activity, antidiarrhoeal activity.

Abstract

The study was aimed to evaluate pharmacological activities of the leaves of Enhydra fluctuans Lour. (Family-Asteraceae). The interesting reported pharmacological activities, coupled with the use of the plant in traditional medicine qualify E. fluctuans Lour. for possible analgesic, antidiarrhoeal and antioxidant activities. These activities were investigated on animal model like Swiss albino mice. Phytochemical analysis using standard screening methods indicated the presence of alkaloids, tannins, steroids, gums, glycosides, saponins and flavonoids in the ethanolic plant extract. The ethanolic extract obtained by cold extraction method, showed anti oxidant activity of IC50 = 197 µgm/ml in DPPH assay while that of the standard ascorbic acid was observed to be IC50 = 18 µgm/ml. In
Folin-Ciocalteau assay total phenolic content was found to be 157 mg GAE/100 gm of dry powder. This extract when applied at 250 and 500 mg/kg exhibits significant inhibition of writhing reflex 25.43% (p< 0.02) and 40.67 % (p< 0.05) respectively while with that of the standard drug diclofenac sodium inhibition was observed to be 68.82% (p<0.001) at a dose of 25 mg/kg body weight. In castor oil induced method the extract produced 23.81 and 47.62 % (p<0.02 and p<0.01) inhibition of diarrhoea at the doses of 250 mg/kg and 500 mg/kg body weight, respectively while standard loperamide showed 85.72 % (p<0.001) inhibition at a dose of 3 mg/kg body weight.

Downloads

Download data is not yet available.

References

Ahmadiani, A.; Hosseiny, J.; Semnanian, S.; Javan, M.; Saeedi, F.; Kamalinejad, M. and Saremi, S. 2000. Antinociceptive and antiinflammatory effects of Elaeagnus angustifolia fruit extract. Journal of Ethnopharmacology, 72: 287–292

Aruoma, O.I. 1994. Nutrition and health aspects of free radicals and antioxidants. Food Chem Toxicol. 32:671–83

Balaji, G.; Chalamaiah, M.; Ramesh, B. and Amarnath, Y.R. 2012. Antidiarrhoeal activity of ethanol and aqueous extracts of Carumcopticum seeds in experimental rats. Asian Pac J Trop Biomed. 2(2):1151–1155

Beecher, G. 2003. Overview of dietary flavonoids: nomenclature, occurrence and intake. J Nutr 133 (10): 3248S–3254S

Chatterjee, T.K. 1993. Handbook of laboratory mice and rats. Calcutta: Jadavpur University, India

Choi, J.; Jung, H.; Lee, K. and Park, H. 2005. Antinociceptive and Antiinflammatory effects of saponin and sapogenin obtained from the stem of Akebia quinata. Journal of Medicinal Food, 8 (1): 78-85

Chopra, R.N.; Chopra, S.L. and Chopra, I.C. 2000. Glossary of Indian Medicinal Plants. New Delhi: NISC (CSIR)

Gupta, B.D.; Dandiva, P.C. and Gupta, M. 1971. A psychopharmacological analysis of behavior in rat. Japanese Journal of Pharmacology 21:293

Horton, E.W.; Main, I.H.M.; Thampson, C.J. and Wright, P.M. 1968. Effects of orally administered PGE on gastric secretion and gastrointestinal motility in man. Gut. 9: 655-658

Izzo, A.A.; Mascolo, N.; Viola, P. and Capasso, F. 1993. Inhibitors of nitric oxide synthase enhance rat ileum contractions induced by ricinoleic acid in vitro. Eur J Pharmacol, 243(1):87–90

Joshi, B.S. and Kamat, V.N. 1972. Structure of enhydrin, a germacronolide from Enhydra fluctuans. Indian Jounal of Chemistry, 10:771–776

Karim, A.; Sohail, M.N.; Munir, S.and Sattar, S. 2011. Pharmacology and phytochemistry of Pakistani herbs and herbal drugs used for treatment of diabetes. Int. J. Pharmacol. 7: 419-439

Kirtikar, K.R. and Basu, B.D. 2002. Enhydra fluctuans. In: Mhaskar, K.S., Cains, J.F.(eds.) Indian Medicinal Plants. Delhi: Sri Satguru Publications

Masuda, T.; Inaba, Y.; Maekawa, T.; Takeda, Y.; Yamaguchi, H. and Nakamoto, K. 2003. Simple detection method of powerful antiradical compounds in the raw extract of plants and its application for the identification of antiradical

plant constituents. Journal of Agricultural and Food Chemistry, 5l : 183l -1838.

Middleton, E.; Kandaswami, C. and Theoharides, T.C. 2000. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease and cancer. Pharmacological Reviews, 52:673–751

Nadkarni, K.M. and Nadkarni, A.K. 2002. Indian Materia Medica. Bombay: Popular Publications

Pierce, N.F.; Carpenter, C.C.J.; Elliot, H.Z. and Greenough, W.B. 1971. Effects of prostaglandins, theophylline and Cholera exotoxin upon transmucosal water and electrolyte movement in canine jejunum. Gastroenterology. 60:22-

Pimiä, R.P.; Nohynek, L.; Meier, C.; Kähkönen, M.; Heinonen, M.and Hopia, A. 2001. Antimicrobial properties of phenolic compounds from berries. Journal of Applied Microbiology. 90:494–507

Podder, M.K.; Das, B.N.; Saha, A. and Ahmed, M. 2011. Analgesic activity of bark of Murraya paniculata. International Journal on Medicine and Medicinal Sciences, 3(4): 105-108

Rajnarayana, K.; Reddy, M.S. and Chaluvadi, M.R. 2001. Bioflavanoids Classification, Pharmacological, Biochemical effects and Therapeutic potential. Indian Journal of Pharmacology, 33: 2-16

Randhir, R.; Lin, Y.T. and Shetty, K. 2004. Phenolics, their antioxidant and antimicrobial activity in dark germinated fenugreek sprouts in response to peptide and phytochemical elicitors. Asia Pacific Journal of Clinical Nutrition, 13:295–307

Sadhu, S. K.; Okuyama, E.; Fujimoto, H. and Ishibashi, M. 2003. Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities. Chemical and Pharmaceutical Bulletin. 51(5): 595-598

Shemsu, U.; Alemu, T.and Nigatu, K. 2013. Antidiarrhoeal and antimicrobial activity of Calpurnia aurea leaf extract. BMC Complement Altern Med. 13:21

Soares, J.R.; Dinis, T.C.P.; Cunha, A.P. and Almeida, L.M. 1997. Antioxidant activities of some extracts of Thymus zygis. Free Rad. Res. 26:469-478

Sofowara, A. 1982. Medicinal Plants and Traditional Medicine in Africa. Johnwilay & sons Limited, New York

Taesotikul, T.; Panthong, A.; Kanjanapothi, D.; Verpoorte, R. and Scheffer, J.J. 2003. Anti inflammatory, antipyretic and antinociceptive activities of Tabernaemontana pandacaqui Poir. J Ethnopharmacol. 84(1): 31-35

Trease, G.E. and Evans, W.C. 1989. Pharmacognosy, 13th Edn., Bailliere Tindale, London

Uddin, S.J.; Ferdous, M.M.; Rouf, R.; Alam, M.S.; Sarkar, M.A.M. and Shilpi JA. 2005. Evaluation of antidiarrhoeal activity of Enhydra fluctuans. Journal of Medicinal Science. 5:324–327

Vital, P.G. and Rivera, W.L. 2011. Antimicrobial activity, cytotoxicity, and phytochemical screening of Voacanga globosa (Blanco) Merr. Leaf extract (Apocynaceae). Asian Pacific Journal of Tropical Medicine. 4(10): 824-828.

Downloads

Published

27-05-2016

How to Cite

[1]
S. . Khushi, M. J. . Akhter, M. M. . Hasan, M. A. . Rahman, G. M. M. . Murshid, and S. K. . Sadhu, “PHYTOCHEMICAL AND PHARMACOLOGICAL SCREENING OF Enhydra fluctuans Lour”., Khulna Univ. Stud., pp. 19–26, May 2016.

Issue

Section

Life Science

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)