IDENTIFICATION OF MUSCLE PROTEIN PATTERN AND GUT PROTEASE ACTIVITY OF ROHU (Labeo rohita), CATLA (Catla catla), CLIMBING PERCH (Anabus testudineus) AND SPOTTED SNAKEHEAD (Channa punctatus) USING SDS-PAGE

Silvia  Ferdous; Md. Shamim  Ahmed; Zerin  Sultana; Md. Monirul  Islam; Md. Ayaz Hasan  Chisty

doi:10.53808/KUS.2010.10.1and2.1004-L

IDENTIFICATION OF MUSCLE PROTEIN PATTERN AND GUT PROTEASE ACTIVITY OF ROHU (Labeo rohita), CATLA (Catla catla), CLIMBING PERCH (Anabus testudineus) AND SPOTTED SNAKEHEAD (Channa punctatus) USING SDS-PAGE

Authors

Silvia Ferdous Fisheries and Marine Resource Technology Discipline (FMRT), Khulna University, Khulna 9208, Bangladesh
Md. Shamim Ahmed Fisheries and Marine Resource Technology Discipline (FMRT), Khulna University, Khulna 9208, Bangladesh
Zerin Sultana Fisheries and Marine Resource Technology Discipline (FMRT), Khulna University, Khulna 9208, Bangladesh
Md. Monirul Islam Fisheries and Marine Resource Technology Discipline (FMRT), Khulna University, Khulna 9208, Bangladesh
Md. Ayaz Hasan Chisty Fisheries and Marine Resource Technology Discipline (FMRT), Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2010.10.1and2.1004-L

Keywords:

casein, enzyme, protein patterns, actin, myosin

Abstract

The study was carried out to identify muscle protein patterns and gut protease activity of rohu (Labeo rohita), catla (Catla catla), climbing perch (Anabas testudineus) and spotted snakehead (Channa punctatus). Twelve percent Polyacryleamide substrate SDS-PAGE was done to visualize the activity of the enzyme after casein hydrolysis and protein pattern of the species. Three alkaline proteases of different molecular weight were observed in rohu, two in catla, five in climbing perch and four in spotted Snakehead from the zymogram. This revealed that the existence of alkaline protein in the alimentary tract of respective species. Fourteen distinct protein bands in rohu, fifteen in catla, sixteen in climbing perch and nineteen in soptted snakehead were visualized from the electrophoretic patterns of fish muscle extract. From the result, it is assumed that heavy molecular weight myosin and low molecular weight actin were prominent in all the experimental species.

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Published

25-11-2010

How to Cite

[1]

S. . Ferdous, M. S. . Ahmed, Z. . Sultana, M. M. . Islam, and M. A. H. . Chisty, “IDENTIFICATION OF MUSCLE PROTEIN PATTERN AND GUT PROTEASE ACTIVITY OF ROHU (Labeo rohita), CATLA (Catla catla), CLIMBING PERCH (Anabus testudineus) AND SPOTTED SNAKEHEAD (Channa punctatus) USING SDS-PAGE”, Khulna Univ. Stud., pp. 161–166, Nov. 2010.