ASSESSMENT OF PHYTOCHEMICAL AND PHARMACOLOGICAL ACTIVITIES OF THE ETHANOLIC EXTRACTS OF Hygrophila spinosa

Authors

  • M.I. Ahmed Pharmacy Discipline, Khulna University, Khulna 9208, Bangladesh
  • M.N. Islam Pharmacy Discipline, Khulna University, Khulna 9208, Bangladesh
  • M.A. Islam Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • N.N. Biswas Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • U.K. Karmakar Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh
  • P. Paul Pharmacy Discipline, Life Science School, Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2010.10.1and2.1009-L

Keywords:

Hygrophila spinosa, analgesic activity, antidiarrhoeal activity, loperamide, diclofenac sodium

Abstract

Ethanolic extract of Hygrophila spinosa plant was prepared and tested for its phytochemical and pharmacological investigation. The extract showed the presence of steroid, alkaloid, reducing sugar, tannin,flavonoids, saponin and glycoside. The extract significantly and dose dependently inhibited the acetic acid induced writhing in mice (58.8%, P<0.001 and 25% at the dose of 500 and 250 mg/kg body weight respectively) and it is comparable to the activity of the standard analgesic drug diclofenac sodium (72.5%, P<0.001 at the dose of 25 mg/kg body weight). The extract of H. spinosa offered about 1.20 hr and 1.8 hr (P<0.001) of the mean latent period for diarrhoeal episode at the doses of 250 and 500 mg/kg body weight which is comparable to the standard antidiarrhoeal drug loperamide. The results tend to suggest that the plant possess analgesic and antidiarrhoeal activities or active constituent(s) responsible for the mentioned activities and thus supported its traditional uses.

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References

Ahmed, F., Selim, M.S.T., Das, A.K., Choudhuri, M.S.K. 2004. Anti-inflammatory and antinociceptive activities of Lipia nodiaflora Linn. Pharmazie 59: 329-330

Beubler, E. and Juan, H. 1979. Effect of Ricinoleic acid and other Laxatives in Net Water Flux and Prostaglandin E release by the Rat Colon, Journal of Pharmacy and Pharmacology 31: 681-685

Chatterjee, T.K. 1993. Handbook of laboratory Mice and Rats. Jadavpur University, India

Deraedt, R., Jouquey, S., Delevallée, F. and Flahaut, M. 1980. Release of Prostaglandins E and F in algogenic reaction and its inhibition. European Journal of pharmacology 51:17-24

Duke, J.A. 1992. Handbook of Biologically Active Phytochemicals and Their Activities. CRC Press, Boca, Raton, FL

Evans, W.C. 1989. Trease and Evan's Text book of Pharmacognosy, 13th edition, Cambridge University Press, London. 546p.

Flavia, C.M., Luiz, A.P., Pizzolatti, M.G., Cândida, A.L., Kassuya, J.B., Adair, R. S. and Santos, A.R. 2006. Analysis of the antinociceptive effect of the Flavonoid Myricitrin: evidence for a role of the

L-arginine-nitric oxide and protein kinase C pathways. The Journal of Pharmacology and Experimental Therapeutics 316:789–796

Ghani, A. 2003. Medicinal Plants of Bangladesh: Chemical constituents and uses. Asiatic Society of Bangladesh, Dhaka

Mazumdar, U.K., Gupta, M., Maiti, S. and Mukherjee, D. 1997. Antitumor activity of Hygrophila spinosa on Ehrlich ascites carcinoma and sarcoma-180 induced mice. Indian Journal of Experimental Biology 35(5): 473-477

Meotti F.C., Fachinetto R., Maffi LC, Missau F.C., Pizzolatti M.G., Rocha J.B. and Santos A.R. 2007. Antinociceptive action of myricitrin: involvement of the K+ and Ca2+ channels. European Journal of Pharmacology 19:567(3):198-205

Racusen, L.C. and Binder, H.J. 1979. Ricinoleic acid stimulation of active anion secretion in colonic mucosa of the rat. Journal of Clinical Investigation 63: 743-749

Taesotikul, T., Panthong, A., Kanjanapothi, D,, Verpoorte, R. and Scheffer, J. J. C. 2003. Anti-inflammatory, antipyretic and antinociceptive activities of Tabernaemontana pandacaqui Poir. Journal of Ethnopharmacology 84(1): 31-35

Whittle B.A. 1964. The use of changes in capillary permeability in mice to distinguish between narcotic and non-narcotic analgesics. British Journal of Pharmacology and Chemotherapy 22: 246-253

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Published

25-11-2010

How to Cite

[1]
M. . Ahmed, M. . Islam, M. . Islam, N. . Biswas, U. . Karmakar, and P. . Paul, “ASSESSMENT OF PHYTOCHEMICAL AND PHARMACOLOGICAL ACTIVITIES OF THE ETHANOLIC EXTRACTS OF Hygrophila spinosa”, Khulna Univ. Stud., pp. 173–178, Nov. 2010.

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