PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF PORTULACA OLERACEA LINN.
DOI:
https://doi.org/10.53808/KUS.2021.18.02.2105-lsKeywords:
Portulaca oleracea, antioxidant activity, phytochemical, DPPH assayAbstract
Portulaca oleracea, an edible herb of Portulacaceae family is widely found in Bangladesh. This plant has many traditional medicinal uses and it is used to treat disorders of urinary, digestive, cardiac, hepatic, renal systems and many diseases. The goal of this research was to search antioxidant potential of crude extract of P. oleracea and its various solvent fragments (water, butanol, chloroform and n-hexane). Phytochemical screening revealed the existence of various phytochemical groups. In DPPH assay, IC50 value of the methanol extract, water, butanol, chloroform fractions were found to be 23, 25, 28 and 41µg/mL respectively. Total flavonoid content of methanol extract and water, butanol and chloroform fractions were found to be 137.72, 102.08, 88.93 and 67.83 mg of QE/gm of arid extract. Methanolic extract, butanol and chloroform fractions exhibited significant reducing power with absorption maximum 1.3121, 1.4231 and 1.4892, respectively. Total content of phenolic of methanolic extract, water, butanol, and chloroform fractions showed 176.70, 157.13, 122.84 and 70.61 mg of GAE/gm of arid extract, respectively. Methanolic extract, butanol and water fractions exhibited significant ferrous ion chelating ability with IC50 value of 25, 32 and 29 µg/mL respectively. Total tannin content of methanol extract and water, butanol, and chloroform fractions showed 140.56, 114.10, 89.14 and 41.44 mg of TAE/gm of arid extract respectively. Methanolic extract, water, butanol and chloroform fragments exhibited significant scavenging ability of nitric oxide (NO) with IC50 10, 27, 24 and 36 µg/mL respectively. Based on these findings, we can say that P. oleracea is a possible reservoir of antioxidants and it can be applied to combat oxidative damages of our body.
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Alam, M.N., Bristi, N.J., Rafiquzzaman, M. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal. 21(2):143-152. https://doi.org/10.1016/j.jsps.2012.05.002
Alam, M.A., Juraimi, A.S., Rafii, M.Y., Hamid, A.A., Aslani, F., Hasan, M.M., Zainuddin, M.A.M., Uddin, K. (2014). Evaluation of antioxidant compounds, antioxidant activities, and mineral composition of 13 collected Purslane. (Portulaca oleracea L.) Accessions. BioMed Research International. Article ID 296063. http://dx.doi.org/10.1155/2014/296063
Aberoumand, A. (2009). Nutritional evaluation of edible Portulaca olera¬cea as plant food. Food Analytical Methods. 2, 204.
Amorim, E.L., Nascimento, J.E., Monteiro, J.M., Peixoto Sobrinho, T., Araújo, T.A., Albuquerque, U.P. (2008). A simple and accurate procedure for the determination of tannin and flavonoid levels and some applications in ethnobotany and ethnopharmacology. Functional Ecosystem and Communities 2(1), 88-94.
Ayoola, G., Coker, H., Adesegun, S., Adepoju-Bello, A., Obaweya, K., Ezennia, E.C., Atangbayila, T. (2008). Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Tropical Journal of Pharmaceutical Research 2008;7(3), 1019-1024. https://doi.org/10.4314/tjpr.v7i3.14686
Bhalodia, N.R., Nariya, P.B., Acharya, R.N., Shukla, V.J. (2013). In vitro antioxidant activity of hydro alcoholic extract from the fruit pulp of Cassia fistula Linn. Ayu. 34(2), 209-214. https://doi.org/10.4103/0974-8520.119684
Boora, F., Chirisa, E., Mukanganyama, S.(2014). Evaluation of Nitrite Radical Scavenging Properties of Selected Zimbabwean Plant Extracts and Their Phytoconstituents. Journal of Food Processing 2014, 918018. http://dx.doi.org/10.1155/2014/918018
Dinis, T.C., Madeira, V.M., Almeida, L.M. (1994). Action of phenolic derivatives (acetaminophen,salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics. 315(1), 161-169. https://doi.org/10.1006/abbi.1994.1485
Fernández-Poyatos, Md.P., Llorent-Martínez, E.J.,. Ruiz-Medina, A. (2021). Phytochemical composition and antioxidant activity of Portulaca oleracea: Influence of the Steaming Cooking Process. Foods, 10, 94. https://doi.org/10.3390/foods10010094
Galati, G., O'brien, P.J. (2004). Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radical Biology and Medicine. 37(3), 287-303. https://doi.org/10.1016/j.freeradbiomed.2004.04.034
Gilgun-Sherki, Y., Melamed, E., Offen, D. (2001). Oxidative stress induced-neurodegenerative diseases: the need for antioxidants that penetrate the blood brain barrier. Neuropharmacology. 40(8), 959-975.
Govindarajan, R., Rastogi, S., Vijayakumar, M., Shirwaikar, A., Rawat, A.K.S., Mehrotra, S., Pushpangadan, P. (2003). Studies on the antioxidant activities of Desmodium gangeticum. Biological and Pharmaceutical Bulletin. 26(10), 1424-1427.
Gupta, M., Mazumdar, U., Sivahkumar, T., Vamis, M., Karki, S., Ramanathan, S., Lakshmanan, M. (2003). Antioxidant and antiinflammatory activities of Acalypha fruticosa. Nigerian Journal of Natural Products and Medicine 7, 25-29. https://doi.org/10.4314/njnpm.v7i1.11700-
Hartmann, T. (2007). From waste products to ecochemicals: fifty years research of plant secondary metabolism. Phytochemistry. 68(22-24), 2831-2846. https://doi.org/10.1016/j.phytochem.2007.09.017
Jin, T., Shen, T., Zhou, M., Li, A., Feng, D., Zheng, B., Gong, J., Sun, J., Li, L., Xiang, L. (2016). Chemical constituents from Portulaca oleracea and their bioactivities. Journal of Chinese Pharmaceutical Sciences 25, 898- 905. http://dx.doi.org/10.5246/jcps.2016.12.101
Kumar, B.S.A., Prabhakarn, V., Lakshman, K., Nandeesh, R., Subramanyam, P., Khan, S., Ranganayakalu, D., Krishna, N.V. (2008). Pharmacognostical studies of Portulaca oleracea Linn. Revista Brasileira de Farmacognosia 18(4), 527-531. http://dx.doi.org/10.1590/S0102-695X2008000400005
Masuda, T., Inaba, Y., Maekawa, T., Takeda, Y., Yamaguchi, H., Nakamoto, K., Kuninaga, H., Nishizato, S., Nonaka, A. (2003). Simple detection method of powerful antiradical compounds in the raw extract of plants and its application for the identification of antiradical plant constituents. Journal of Agricultural and Food Chemistry. 51(7), 1831-1838.
https://doi.org/10.1021/jf026112m
Moreno, M.a.I.N., Isla, M.a.I., Sampietro, A.R., Vattuone, M.A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology. 71(1-2), 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
Nabavi, S.M., Ebrahimzadeh, M.A., Nabavi, S.F., Fazelian, M., Eslami, B. (2009). In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Pharmacognosy Magazine. 5(18), 122.
Okolie, N.P., Falodun, A., Davids, O. (2014). Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetia tripetala), and it’s potential for the inhibition of lipid peroxidation. African Journal of Traditional, Complementary and Alternative Medicines. 11(3), 221-227. http://dx.doi.org/10.4314/ajtcam.v11i3.31
Olusina, O.K., Adeoti, A., Abayomi, O., Kolapo, A. (2016). Recovery of reproductive functions in male rats treated with crude extracts of Portulaca oleracea. International Journal of Pharmaceutical Sciences Review and Research. 38, 237-241. http://dx.doi.org/10.4314/ajtcam.v11i3.31
Palaniswamy, U.R., McAvoy, R.J., Bible, B.B. (2001). Stage of harvest and polyunsaturated essential fatty acid concentrations in purslane (Portulaca oleraceae) leaves. Journal of Agricultural and Food Chemistry. 49(7), 3490-3493. https://doi.org/10.1021/jf0102113
Rahman, S., Sarder, M., Shilpi, J.A., Hasan, C.M. (2006). Antioxidant and analgesic activity of Clerodendrum visconsum leaf. Oriental Pharmacy and Experimental Medicine. 6(4), 319-323. https://doi.org/10.3742/OPEM.2006.6.4.319
Rogers, N.M., Seeger, F., Garcin, E.D., Roberts, D.D., Isenberg, J.S. (2014). Regulation of soluble guanylate cyclase by matricellular thrombospondins: implications for blood flow. Frontiers in Physiology. 5, 134. https://doi.org/10.3389/fphys.2014.00134
Sadhu, S.K., Okuyama, E., Fujimoto, H., Ishibashi, M. (2003). Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities. Chemical and Pharmaceutical Bulletin. 51(5), 595-598. https://doi.org/10.1248/cpb.51.595
Samydurai, P., Thangapandian, V. (2012). Antioxidant property and polyphenols evaluation of aqueous root extract of Decalepis hamiltonii Wight & Arn. International Current Pharmaceutical Journal. 1(4), 71-76. https://doi.org/10.3329/icpj.v1i4.10061
Sicari, V., Loizzo, M.R., Tundis, R., Mincione, A., Pellicanò, T.M. (2018). Portulaca oleracea L. (Purslane) extracts display antioxidant and hypoglycaemic effects. Journal of Applied Botany and Food Quality. 91, 39 – 46. https://doi.org/10.5073/JABFQ.2018.091.006
Sudan, R., Bhagat, M., Gupta, S., Singh, J., Koul, A. (2014). Iron (Fe II) Chelation, Ferric Reducing, Antioxidant Power, and Immune Modulating Potential of Arisaema jacquemontii (Himalayan Cobra Lily). BioMed Research International. 179865. https://doi.org/10.1155/2014/179865
Wolfe, K., Wu, X., Liu, R.H. (2003). Antioxidant activity of apple peels. Journal of Agriculture and Food Chemistry. 51(3), 609-614. https://doi.org/10.1021/jf020782a
Zhou, Y.-X., Xin, H.-L., Rahman, K., Wang, S.-J., Peng, C., Zhang, H. (2015). Portulaca oleracea L.: a review of phytochemistry and pharmacological effects. BioMed Research International, 2015:925631.
https://doi.org/10.1155/2015/925631
Zuo, L., Zhou, T., Pannell, B., Ziegler, A., Best, T.M., (2015). Biological and physiological role of reactive oxygen species–the good, the bad and the ugly. Acta Physiologica. 214(3), 329-348. https://doi.org/10.1111/apha.12515
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