• Md. Shamim Gazi Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh.
  • Khondoker Moazzem Hossain Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh.
  • Begum Rokeya Department of Pharmacology, Bangladesh University of Health Sciences, Mirpur-1, Dhaka-1216, Bangladesh.
  • Pallob Barai Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh.
  • Md. Faysal Al Mazid Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh.




Diabetes, rats, probiotics, yogurt, metabolites


Probiotics can be considered as biological agents that modify the intestinal microbiota as well as show several health benefits. The purpose of this study was to explore the effects of probiotics in neonatal STZ-induced type-2 diabetic rats. Following the biochemical analysis of probiotic bacteria, yogurt was prepared using cow milk and used to feed the experimental rats. Probiotic yogurt was supplemented to three rat groups, each with six rats, and the doses were 2 g in PYT1, 4 g in PYT2 and 6 g in PYT3 for 21 days. Fasting serum glucose, serum insulin, lipid profiles and liver glycogen levels were measured to investigate the probiotic effects on type 2 diabetic rats. It was observed that fasting serum glucose levels were significantly lower in case of PYT3 group (p<0.05) whereas a significant (p<0.05) upsurge of serum insulin levels was detected in PYT2 rat group. A significant (p<0.05) decrease in LDL level in PYT2 group and significantly (p<0.05) increased level of HDL was detected in PYT1 and PYT2 rat groups. However, there were no significant differences regarding triglyceride as well as total cholesterol levels among three probiotic yogurt supplemented rat groups. Hepatic glycogen content was 34.5%, 30.9% and 39.1% among the probiotic yogurt feed groups viz. PYT1, PYT2 and PYT3, respectively. Findings of this research suggest that application of probiotic yogurt can help to manage type-2 diabetes. Further study on clinical trial would be worthy to investigate for probiotic-based product improvement for treatment of type-2 diabetic patients.


Download data is not yet available.


Abbas, Z., & Jafri, W. (1992). Yogurt (dahi): a probiotic and therapeutic view. Journal of Pakistan Medical Association, 42(9), 221.

Al Azad, S., Moazzem Hossain, K., Rahman, S. M. M., Al Mazid, M. F., Barai, P., & Gazi, M. S. (2020). In ovo inoculation of duck embryos with different strains of Bacillus cereus to analyse their synergistic post‐hatch anti‐allergic potentialities. Veterinary medicine and science, 6(4), 992-999.

Andersson, U., Bränning, C., Ahrné, S., Molin, G., Alenfall, J., Önning, G., & Holm, C. J. B. M. (2010). Probiotics lower plasma glucose in the high-fat fed C57BL/6J mouse. Beneficial microbes, 1(2), 189-196.

Asrafuzzaman, M., Rahman, M. M., Mandal, M., Marjuque, M., Bhowmik, A., Rokeya, B., & Faruque, M. O. (2018). Oyster mushroom functions as an anti-hyperglycaemic through phosphorylation of AMPK and increased expression of GLUT4 in type 2 diabetic model rats. Journal of Taibah University medical sciences, 13(5), 465-471.

Ataie-Jafari, A., Larijani, B., Majd, H. A., &Tahbaz, F. (2009). Cholesterol-lowering effect of probiotic yogurt in comparison with ordinary yogurt in mildly to moderately hypercholesterolemic subjects. Annals of Nutrition and Metabolism, 54(1), 22-27.

Barai, P., Hossain, K. M., Rahman, S. M. M., Al Mazid, M. F., & Gazi, M. S. (2018). Antidiarrheal efficacy of probiotic bacteria in castor oil induced diarrheal mice. Preventive Nutrition and Food Science, 23(4), 294. Barik, R., Jain, S., Qwatra, D., Joshi, A., Tripathi, G. S., & Goyal, R. (2008). Antidiabetic activity of aqueous root extract of Ichnocarpus frutescens in streptozotocin-nicotinamide induced type-II diabetes in rats. Indian Journal of Pharmacology, 40(1), 19.

Brady, L. J., Gallaher, D. D., &Busta, F. F. (2000). The role of probiotic cultures in the prevention of colon cancer. The Journal of nutrition, 130(2), 410S-414S.

Chawla, K., &Kansal, V. K. (1984). Effect of milk & its culture products on the plasma & organ lipids in rats. The Indian Journal of Medical Research, 79, 418-425.

Chen, J., Wang, R., Li, X. F., & Wang, R. L. (2012). Bifidobacterium adolescentis supplementation ameliorates visceral fat accumulation and insulin sensitivity in an experimental model of the metabolic syndrome. British Journal of Nutrition, 107(10), 1429-1434.

Cho, N., Shaw, J. E., Karuranga, S., Huang, Y., da Rocha Fernandes, J. D., Ohlrogge, A. W., & Malanda, B. (2018). IDF Diabetes Atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045. Diabetes research and clinical practice, 138, 271-281.

Chou, L. S., & Weimer, B. (1999). Isolation and characterization of acid-and bile-tolerant isolates from strains of Lactobacillus acidophilus. Journal of Dairy Science, 82(1), 23-31.

Collado, M. C., Meriluoto, J., &Salminen, S. (2007). Role of commercial probiotic strains against human pathogen adhesion to intestinal mucus. Letters in applied microbiology, 45(4), 454-460.

Delia, E., Tafaj, M., &Männer, K. (2012). Efficiency of probiotics in farm animals. Probiotic in animals, 247-272.

Fossati, P., &Prencipe, L. (1982). Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical chemistry, 28(10), 2077-2080.

Fridewald, W. T. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem, 18, 499-502.

Ghanbari, M., Rezaei, M., Jami, M., & Nazari, R. M. (2009). Isolation and characterization of Lactobacillus species from intestinal contents of beluga (Huso huso) and Persian sturgeon (Acipenser persicus).

Hasan, M. N., Sabrin, F., Rokeya, B., Khan, M. S. H., Ahmed, M. U., Matondo, A., & Akter, S. (2019). Glucose and lipid lowering effects of Enhydra fluctuans extract in cadmium treated normal and type-2 diabetic model rats. BMC complementary and alternative medicine, 19(1), 1-10.

Hassan, M., Islam, M., Uddin, S., Bhowmik, A., &Rokeya, B. (2018). Antihyperglycemic Potential of Ethanolic Extract of Couroupitaguianensis on Streptozocin Induced Experimental Diabetic Rat Model. Asian Journal of Research in Medical and Pharmaceutical Sciences, 5(3), 1-10.

He, C., Shan, Y., & Song, W. (2015). Targeting gut microbiota as a possible therapy for diabetes. Nutrition Research, 35(5),361-367. Hoque, M. Z., Akter, F., Hossain, K. M., Rahman, M. S. M., Billah, M. M., & Islam, K. M. D. (2010). Isolation, identification and analysis of probiotic properties of Lactobacillus spp. from selective regional yogurts. World J Dairy Food Sci, 5(1), 39-46. Hossain, K. M., Barai, P., Rahman, S. M. M., Al Mazid, M. F., Gazi, M. S., & Jalil, M. A. (2018). Isolation and biochemical characterization of probiotic bacteria obtained from yogurt samples of Rajshahi and Chittagong divisions of Bangladesh and their antimicrobial activity against enteric pathogens. Bangladesh Journal of Livestock Research, 142-152.

Federation, I. D. (2019). IDF Diabetes Atlas, 9th edn. Brussels, Belgium; 2019. Kendall, F. E. (1952). A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J biol Chem, 195, 357-366. Larsen, N., Vogensen, F. K., Van Den Berg, F. W., Nielsen, D. S., Andreasen, A. S., Pedersen, B. K., ... & Jakobsen, M. (2010). Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PloS one, 5(2), e9085. Lu, Y. C., Yin, L. T., Chang, W. T., & Huang, J. S. (2010). Effect of Lactobacillus reuteri GMNL-263 treatment on renal fibrosis in diabetic rats. Journal of bioscience and bioengineering, 110(6), 709-715. Markowiak, P., &Śliżewska, K. (2017). Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients, 9(9),1021.

Morelli, L., &Capurso, L. (2012). FAO/WHO guidelines on probiotics: 10 years later. Journal of clinical gastroenterology, 46,S1-S2. Mudi, S. R., Akhter, M., Biswas, S. K., Muttalib, M. A., Choudhury, S., Rokeya, B., & Ali, L. (2017). Effect of aqueous extract of Aegle marmelos fruit and leaf on glycemic, insulinemic and lipidemic status of type 2 diabetic model rats. Journal of Complementary and Integrative Medicine, 14(2).

O'Shea, E. F., Cotter, P. D., Stanton, C., Ross, R. P., & Hill, C. (2012). Production of bioactive substances by intestinal bacteria as a basis for explaining probiotic mechanisms: bacteriocins and conjugated linoleic acid. International journal of food microbiology, 152(3), 189-205.

Ortiz-Martínez, M., González-González, M., Martagón, A. J., Hlavinka, V., Willson, R. C., & Rito-Palomares, M. (2022). Recent Developments in Biomarkers for Diagnosis and Screening of Type 2 Diabetes Mellitus. Current Diabetes Reports, 1-21. Prasad, J., Gill, H., Smart, J., & Gopal, P. K. (1998). Selection and characterisation of Lactobacillus and Bifidobacterium strains for use as probiotics. International Dairy Journal, 8(12), 993-1002.

Reid, G., Anand, S., Bingham, M. O., Mbugua, G., Wadstrom, T., Fuller, R., Anukam, K., &Katsivo, M. (2005). Probiotics for the developing world. Journal of clinical gastroenterology, 39(6), 485–488.

Riezzo, G., Chiloiro, M., & Russo, F. (2005). Functional foods: salient features and clinical applications. Current Drug Targets-Immune, Endocrine & Metabolic Disorders, 5(3), 331-337.

Ruan, Y., Sun, J., He, J., Chen, F., Chen, R., & Chen, H. (2015). Effect of probiotics on glycemic control: a systematic review and meta-analysis of randomized, controlled trials. PloS one, 10(7), e0132121.

Spinler, J. K., Taweechotipatr, M., Rognerud, C. L., Ou, C. N., Tumwasorn, S., &Versalovic, J. (2008). Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe, 14(3), 166-171. Stenvers, D. J., Scheer, F. A., Schrauwen, P., la Fleur, S. E., &Kalsbeek, A. (2019). Circadian clocks and insulin resistance. Nature Reviews Endocrinology, 15(2), 75-89.

Sunday, H. G., Sadia, A. H., & Ojo, O. G. (2022). Mechanisms of Diabetes Mellitus Progression: A Review. Journal of Diabetic Nephropathy and Diabetes Management, 1(1), 1-5.

Trinder, P. (1969). Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. Journal of clinical pathology, 22(2), 158-161.

Vries, J.V., 1954. Two methods for the determination of glycogen in liver. Biochem. J., 57: 410-416.

Walker, W. A. (2000). Role of nutrients and bacterial colonization in the development of intestinal host defense. Journal of pediatric gastroenterology and nutrition, 30, S2-S7.

Xanthopoulos, V., Litopoulou-Tzanetaki, E., &Tzanetakis, N. (2000). Characterization of Lactobacillus isolates from infant faeces as dietary adjuncts. Food Microbiology, 17(2), 205-215.

Yadav, H., Jain, S., & Sinha, P. R. (2007). Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei in high fructose fed rats. Nutrition, 23(1), 62-68.

Yadav, H., Jain, S., & Sinha, P. R. (2008). The effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei on gastropathic consequences in diabetic rats. Journal of medicinal food, 11(1), 62-68.

Yun, S. I., Park, H. O., & Kang, J. H. (2009). Effect of Lactobacillus gasseri BNR17 on blood glucose levels and body weight in a mouse model of type 2 diabetes. Journal of applied microbiology, 107(5), 1681-1686.




How to Cite

M. S. . Gazi, K. M. Hossain, B. . Rokeya, P. . Barai, and M. F. . Al Mazid, “EFFECTS OF PROBIOTIC SUPPLEMENTED YOGURT ON NEONATAL STREPTOZOTOCIN-INDUCED TYPE-2 DIABETIC RATS”, Khulna Univ. Stud., pp. 57–65, Dec. 2022.



Life Science