SHORT-TERM CONSERVATION OF SWEET POTATO ( Ipomoea batatas L.) IN VITRO

R.  Alam; S. M. M.  Rahman; M. N.  Amin

doi:10.53808/KUS.2002.4.1.0224-L

Authors

R. Alam Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.
S. M. M. Rahman Biotechnology Discipline, Khulna University, Khulna-9208. Bangladsh.
M. N. Amin Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

DOI:

https://doi.org/10.53808/KUS.2002.4.1.0224-L

Keywords:

Sweet potato. Auxin, Cytokinin, Medium and Preservation.

Abstract

An Investigation was carried out to develop the techniques for short-term in vitro conservation using shoot tip and nodal explant of Ipomoea batatas. The culture conditions were 25-27°C room temperature under dark or light and refrigerator temperature 10-12°C under dark. The explants which were preserved on agae-sucrose-1/4MS medium at refrigerator (10-12#C) under dark condition showed the highest survival rates (60 & 70%) for shoot tip and nodal explants for variety ‘white skinned' while 63 & 77% for ‘red skinned' variety, respectively than other media (agarsucrose medium and agar-sucrose-MS medium). For the test of resumption of growth of the in vitro preserved explants which were survived on preservation media for 3 months and 6 months conservation period, were cultured on MS medium supplemented with 1.0 mg/l BA (proliferation medium ) at 25-27°C under light. It was evident that, the explant those were preserved at 25-27°C under light were more responsive than those were preserved at refrigerator (10-12°C) under dark for resumption of growth.

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References

Bajaj, Y.P.S. (1986). In vitro preservation of genetic resources-techniques and problems. In: Nuclear techniques and in vitro culture of plant improvement, IEAE-SM-282/66, Vienna, pp. 43-57.

Kruik, N., A. Supat and S. Kasem (1990). In vitro germplasm preservation and exchange of economic plants. In: Proceeding, Use of biotechnology forilmproving rich and other plants. National Center for Genetic Engineering and Biotechnology, Bangkok, pp. 1-2.

Lai, N. and P.S. Ahuja (1995). Establishment of short-term tissue cultures of Picrorhiza korroa and Rheum emodi. Plant Tissue Culture. 63: 1-9.

Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bioassays with tobacco cultures.Plant Physiology. 15: 473-497.

Ng, S.Y.C. (1991). In vitro conservation and distributuin of root and tuber crop germplasm. In: Proceedings, International Symposium. Crop Genetic Resources, Africa, 1ITA, PMD 5320, Nigeria, pp. 119-128.

Samsuddin, S.A.K. (1983). Studies on physiological factors affecting storage root formation of sweet potato( Ipomoea batatas L.) and its relationship with photosynthetic area, JIF. Phill thesis, Department of Botany, University of Rajshahi. 270 pp.

Sharfuddin, A.F.M. and V. Voican (1985). Studies on the effects of plant density and NPK dose on the chemical composition of fresh and stored sweet potatoes. Bangladesh Journal of Agricultural Research. 10: 23-27.

Zakir, A.H. M.N. Norman, A.G.A. Karim and S. Senawi (1990). Conservation of plant geneti resources through in vitro methods, PRIM & MNCPGR, Kualalampur. 271 pp.