COMPARATIVE SERO EVALUATION OK LIVE AND KILLED NEWCASTLE DISEASE (ND) VACCINE IN BROILER FARM

K. M.  Hossain; M. M.  Alam; B. K.  Sil; M.J.F.A.  Taimur

doi:10.53808/KUS.2002.4.1.0211-L

Authors

K. M. Hossain Biotechnology Discipline, Khulna University, Khulna-9208. Bangladsh.
M. M. Alam Biotechnology Discipline, Khulna University, Khulna-9208. Bangladsh.
B. K. Sil Bangladesh Livestock Research Institute. Savar. Dhaka. Bangladesh.
M.J.F.A. Taimur Bangladesh Livestock Research Institute. Savar. Dhaka. Bangladesh.

DOI:

https://doi.org/10.53808/KUS.2002.4.1.0211-L

Keywords:

Live & Killed Newcastle Disease Vaccine, ELISA, Heamoagglutination Inhibition test.

Abstract

Qualitative and quantitative measurements of antibody level in serum : nated broiler flock (120 broilers), batch namc-"broiler-43" of Usha Poultry and Feed Farm was performed :n - r_ urd to compare the immunoresponse of both live and killed Newcastle Disease (ND) vaccine. Haemoagglu'.irution inhibition . HI • and enzyme-linked immunosorbent assay (ELISA) tests were performed. To achieve this task. m sennr. sampling was done. The tests were performed at the Virology Laboratory of Bangladesh Livestock Research Institute BLR! . Sa\ ar.Dhaka, and Usha Poultry and Feed Farm Immunology Laboratory. Twelve serum samples were collected of which 10
were tested using both HI and ELISA tests, respectively. The average hacmoagglutination count was 1 12 arc FI ISA titer was 3273 when flocks vaccinated at day 7. Tests were performed after 6 day of vaccine administration The rest of the tests were performed 14 days after vaccine administration. The result in regard to immune response vaccinated a: day 21 showed HI average 30 and ELISA average 598. The subsequent results when vaccinated at day 35 showed HI titer average 58 and ELISA titer average 2264. Analytical results when vaccinated at day 70 showed average HI titer 128 and ELISA titer 4311. When vaccinated at day 120, results showed HI titer average 480 and ELISA titer average 8122. The nature of immunoresponse was increasing at the beginning, decreasing at the mid-point and again increasing later or. Live vaccine showed short term high immunoresponse whereas, killed vaccine showed long term slow' increasing immunoresponse. Further works on sero-response on molecular analysis of immunity would be worthy to investigate.

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