TOXICOLOGICAL STUDIES OF ANTIBIOTICS FROM A STRPTOMYCES STRAIN ON LONG EVAN’S RATS

M. M.  Masud; A.  Jabbar

doi:10.53808/KUS.2002.4.1.0148-L

Authors

M. M. Masud Pharmacy Discipline, Khulna University, Khulna-9208, Bangladesh
A. Jabbar Department of Pharmacy, University of Dhaka, Dhaka-WOO, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2002.4.1.0148-L

Keywords:

Toxicological study, Antibiotic, Streptomyce

Abstract

The sub-acute toxicity studies of two antibiotics (S, & SJ, isolated from the culture filtrate of a Streptomyces strain were carried out on long evan's rats. The study included the gross general observation, body weight changes, hematological profiles, such as, total count of RBC & WBC. differential count of WBC, platelet count & hemoglobin percentage, biochemical parameters of blood, such as, SGOT, SGPT, serum alkaline phosphate, serum bilirubin, serum uric acid & scrum urea, and histopathological studies of liver, kidney, heart, lungs & spleen tissues. The antibiotics did not show any abnormalities on the hematological profiles. The body weight and biochemical parameters of blood were found to be slightly changes with respect to that of control but remained within the normal range and statistically insignificant. No detectable abnormalities were observed in the histopathological studies

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References

Coulombe J J and Favreau L (1963) A new simple semimicro method for colorimetric determination of urea. J. Clin. Chem. 9: 102-108.

Fawcett J K and Scott J E (1960) A new simple semimicro method for the determination of urea. J. Clin. Path. 13: 156-159.

Hawk P, Oser L and Summerson W H (1954) Practical Physiological Chemistry. 13th edition, McGraw Hill Co. USA.

King P J E and Armstrong A R (1934) A convenient method for determination serum and bile phosphate activity. Cand. Med. Asso. 31: 376-381.

Masud, M. M. (1997). Antibiotics from a Streptomyces strain. M. Pharm. thesis, Department of Pharmacy, University of Dhaka, Bangladesh.

Marston A and Hostettmam K (1991) Modem separation methods. Natural product reports.391-393 pp. New challenge of infectious diseases. (1994). London School of Hygine and Tropical Medicine News Letter. Latimer Trend and Company Ltd. Plymouth.

Reitman S and Frankel S (1957) A colorimetric method for determination of serum glutamate-oxaloacetate and glutamate-pyruvate transaminase. Am. J. Clin. Path. 28: 56-63.

Schatz, A.; Bugie, S. and Waksman, S.A. (1944). Streptomycin, a substance exhibiting antibiotic activity against gram positive and gram negative bacteria. Proc. Soc. Exp. Biol. Med.,57,244-248.

Tabata N, Tomoda H, Iwai Y and Omura S (1995). Fudecalone, a new anticoccidial agent produced by Penicillium sp. FO- 2295. J. Antibiot. Tokyo. 48: 53-58.

Waksman, S. A. and Woodruff, H.B. (1940). Bacteriostatic and bactericidal substances produced by a soil Actinomycetes. Proc.Soc. Exp. Biol. Med.,45, 609-614.