EFFECTS OF DIETARY PROTEIN UPON CONCENTRATIONS OF UREA IN PERIPHERAL PLASMA AND UTERINE FLUID IN DAIRY CATTLE

Authors

  • K.M. Hossain Biotechnology Discipline, Khulna University, Khulna-9208. Bangladsh.
  • N.P. McMeniman Animal Health and Production Department, University of Queensland, QLD. 4072, Australia.

DOI:

https://doi.org/10.53808/KUS.2001.3.2.0116-L

Keywords:

Crude Protein, Blood & Uterine Urea-N and Ammonia in Dairy Cattle

Abstract

There have been concerns that elevated urea nitrogen concentrations in uterine fluid had significant effects on bovine spermatozoal motility and survival. However, it is still uncertain whether dietary protein has any effects upon concentration of urea nitrogen and ammonia in the peripheral plasma and uterine fluid in dairy cattle. The objectives of the study were to investigate the effects of dietary protein intake on concentrations of (i) urea nitrogen in the uterine fluid, (ii) ammonia in the blood and (iii) urea nitrogen in the peripheral plasma. Six lactating, non-pregnant and normally cycling Friesian cows were used in two randomly divided equal groups, receiving either a low or high dietary protein intake. Uterine fluids and blood samples were collected from each cow and uterine urea nitrogen; blood ammonia and plasma urea nitrogen concentrations were measured. The study showed that the cows fed the high protein (HP) diet had significantly higher uterine urea nitrogen, blood ammonia and plasma urea nitrogen concentrations than cows fed the low protein (LP) diet. The results revealed that the concentration of urea nitrogen in the uterine fluid were higher in cows fed HP diet compared with those fed a LP diet. These results suggested that high concentrations of urea in the plasma could diffuse into the uterine lumen and might create a sub-optimal environment in the uterus, which might act as an antagonist to fertility. The high level of blood ammonia and plasma urea nitrogen concentration in the HP fed cows, which could occur due to high protein intake, may affect the viability of sperm, ova and embryos and the growth and survival of the foetus by producing biochemical, endocrionological and tissue derangement. Therefore, it is likely that excessive concentration of urea nitrogen in peripheral plasma and uterine fluid and plasma ammonia of high protein fed lactating cows may be detrimental to the viability of sperm, ova, embryos or foetus.

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References

Carroll, D.J., Barton, B.A., Anderson, G.W. & Smith, R.D. 1988. Influence of protein intake and feeding strategy on reproductive performance of dairy cows. J. Dairy Sci.71, 3470-3481.

Chalupa, W. 1974. Discussion of protein symposium. J. Dairy Sci. 67, 1134-1146.

Dasgupta, P.R., Kar, A.B. & Dhar, M.I. 1971. Spermicidal activity of urea. Indian J. Exp. Biol. 9, 414-415.

Edwards. J.S., Bartley, E.E. & Dayton, A.D. 1980. Effects of dietary protein concentration on lactating cows. J. Dairy Sci.63, 243-248.

Ferguson, J.D., Blanchard, T. & Chalupa, W. 1986. The effects of protein level and type on reproduction in the dairy cow. In Proc.Soc. Theriogenology Ann. Mtg., Rochester, NY. pp. 164-185, Soc. Theriogenology, Hastings, NE.

Ferguson, J.D., Blanchard, T., Galligan, D.T., Hoshal, D.C. & Chalupa, W. 1988. Infertility in dairy cattle fed a high percentage of protein degradable in the rumen. J Am. Vet., Med. Assoc. 192, 659-662.

Gould, C.M 1969. Unifying hypothesis-fertility status, palatability, ketosis, and protein intake. Vet. Rec.85 662.

Haour, R. & Saxena, B.B. 1974. Characterization and solubilization of gonadotropin receptor of bovine corpus luteum. J. Biol. Chem. 249, 2195-2205.

Heap, R.B. 1982. Some chemical constituents of uterine washings: a method of analysis with results from various species. J. Endocr. 24, 367-378.

Hossain, K.M., N.P. McMeniman, K.F. Dowselt, D. Jillella & L. M. Knott. 1998. The effects of dietary crude protein, urea solutions and uterine urea concentrations upon sperm motility and survival. B.J. of Livestock Research. Vol. 1 (2) & 5(2), 72-91.

Howards, S.S., lessee, S.J., & Johnson A.L. 1976. Micropuncture studies of the blood-seminiferous tubule barrier. Biol. Reprod. 14, 264-269.

Johanson, C.E., & Woodbury, D.M. 1978. Upatke of [14C]-urea by the in vivo choroid plexuscerebrospinal fluid-brain system; identification of sites of molecular sieving. J. Physiol.,Land. 275,167-176.

Jordan, E.R & Swanson, L.V. 1979a. Effect of crude protein on reproductive efficiency, serum total protein and albumin in the high producing dairy cow. J.Dairy Sci.62, 58-63.

Jordan, E.R., Chapman, T.E., Holtan, D.W. & Swanson, L.V. 1983. Relationship of dietary crude protein to composition of uterine secretions and blood in high-producing postpartum dairy cows. J. Dairy Sci. 66. 1854-1862.

McRae, A.C. & Kennedy, T.G. 1981. Oestrogen, progesterone and the blood-uterine lumen permeability barrier in rats. Biol. Reprod. 25, 314-320.

Norton, J.H., Tranter, W.P., Campbell, R.S.F. & Shepherd, R.K. 1989. A farming systems study of abortion in dairy cattle on the Atherton Tableland: 3, metabolic factors. Aust. Vet. J. 66, 167-170.

Pycock, J.F. & Allen, W.E. 1990. Inflammatory components in uterine fluid from mares with experimentally induced bacterial endometritis. Equine Vet. J.22, 422-425.

Visek, W.J. 1984. Ammonia: its effects on biological systems, metabolic hormones and reproduction. J. Dairy Sci.67, 481-498.

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Published

28-11-2001

How to Cite

[1]
K. . Hossain and N. McMeniman, “EFFECTS OF DIETARY PROTEIN UPON CONCENTRATIONS OF UREA IN PERIPHERAL PLASMA AND UTERINE FLUID IN DAIRY CATTLE”, Khulna Univ. Stud., pp. 533–536, Nov. 2001.

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Section

Life Science

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