Effect of Different Media Additives on In vitro Culture of Potato (Solanum tuberosum)

S.A.K.U.  Khan; M.G.  Rabbani

doi:10.53808/KUS.2001.3.1.0128-L

Authors

S.A.K.U. Khan Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
M.G. Rabbani Department of Horticulture, Bangladesh Agricultural University, Mymensingh - 2202

DOI:

https://doi.org/10.53808/KUS.2001.3.1.0128-L

Keywords:

Growth rate, In vitro culture, Media additives, Growth regulator, Potato.

Abstract

Effect of different media additives on in vitro culture of three potato varieties viz.Lalpakri, Cardinal and BARI TPS-1 was investigated using single nodes as explant. The single nodes were collected from microplants of tuber sprouts that was preincubated under dark. Survivability of the cultures was statistically insignificant in respect of the media used, but the highest percentage of cultures survived on MS medium supplimented with 60 mg L-1 inositol. The time taken to initiate new shoots was minimum on MS medium supplemented with 1 mg L-1 Benzyle Amino Purine (BAP). The number of shoot was maximum on a medium containing MS + 0.25 mg L-1 Gibberellic Acid (GA3) and 2 mg L-1 Calcium Pantothenate. The maximum number of roots was obtained on medium containing MS + 0.03 mg L-1 Naphthalene Acetic Acid (NAA). Among the varieties tested, BARI TPS-1 performed better in respect of single node culture. The interaction between different potato varieties and media compositions was significant for different characters of in vitro grown plantlets.

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References

Bajaj, Y.P.S., 1981. Regeneration of plants from potato meristem freeze-preserved for 24 months. Euphytica 30:141-145.

Chavdarov, C.B., Dimitrova, D. and Ruseva, R., 1983. The effect of 3-indoleacetic and indolebutyric acids on growth and root formation of in vitro produced potato plants. Potato Abstract, 9: 149.

FAO (Food and Agricultural Organization)., 1995. Production Yearbook. Food and Agricultural Organization of the United Nations. Rome, 49:89.

Firmin, D.M., 1984. Gibbrellic acid as media additive in vitro propagation of potato. Journal of Agricultural Science 103:703-704.

Gomez, R.A. and Gomez, A.A., 1984. Statistical Procedure for Agricultural Research (2nd ed.). International Rice Research Institute. A Willey Interscience Pub., pp. 28-192.

Heszky, L.E., Enyingi, K. and Szabo, I., 1983. Tissue culture technology for long-term storage and propagation of potato germplasms. In: S.K. Sen and Gibs, K.L. (eds.), Plant Cell Culture in Crop Improvement, Planum Publications, New York. pp. 9-17.

Hoque, M.I., Mila, N.B., Khan, M.S. and Sarker, R.H., 1996. Shoot regeneration and in vitro microtuber formation in potato (Solanum tuberosum). Bangladesh Journal of Botany. 25:87-93.

Hossain, M.J., 1988. In vitro node culture of potato. Bangladesh Horticulture, 16 : 1-7.

Hussey, G., 1980. In vitro propagation. In: Ingram, D.S. and Helgeson, J.P. (eds.), Tissue culture methods for plant pathologists. Blackwell Scientific Publications. Oxford. pp. 51-61.

Hussey, G. and Stacey, N.J., 1981. In vitro propagation of potato (Solanum tuberosum). Annals of Botany. 48:787-796.

Kollist, Y.U. and Tikke, E., 1994. Relationship of callus regeneration in potato to the conditions in induction. Potato Abstract, 21:1136.

Letal, J.R., Salapek, S. and Davies, W., 1985. Propagation of seed potatoes by tissue culture techniques. Annual Report of Horticulture Research Centre, Canada. Potato Abstract., 12: 373.

Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacoo tissue culture. Physiology of Plant 15:473-497.

Obordo, J.A.S. and Zamora, A.B., 1988. In vitro tuberization in white potato. Phillipine Journal of Crop Science. 13: 543.

Resende, R.D.O. and Paiva, M., 1986. In vitro meristem culture of potato. Potato Abstract. 14:986.

Roca, W.M., Bryan, J.E. and Roca, M., 1979. Tissue culture for the international transfer of potato genetic resources, American Potato Journal. 56: 694-701.

Smolenskaya, S.E., 1989, Study of features of shoot formation in callus tissue culture. Potato Abstract, 15: 1105.