IN VITRO REGENERATION OF PROMISING SUGARCANE VARIETIES OF BANGLADESH

Authors

  • S.M.M. Rahman Biotechnology Discipline, Khulna University, Khulna-9208. Bangladsh.
  • M.A. Mamun Biotechnology Discipline, Khulna University, Khulna-9208. Bangladsh.
  • M.Z. Karim Bangladesh Sugarcane Research Institute, lshurdi-5205, Bangladesh
  • A.B.M.M. Rahman Bangladesh Sugarcane Research Institute, lshurdi-5205, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2001.3.1.0146-L

Keywords:

Sugarcane, Surface sterilization, Auxins, Cytokinin and Callus

Abstract

In vitro regeneration of sugarcane varieties viz. Isd-20, Isd-30 and Isd-2/54 has been studied. Nutrient media containing growth regulators enhanced callus induction, shoot differentiation and root formation in vitro. All the three varieties cultured fortified with 3.0 mg/1 2,4-D, showed the best performance in the callus induction on modified MS media. In shoot induction, effectiveness of cytokinin (BA 1.5 mg/1) along with either 0.25 mg/1 NAA or IBA was proved to be superior to other concentrations. Microshoots were rooted on MS and modified MS media. The striking number of roots was observed on modified MS medium supplemented with NAA 0.5 mg/1 + 0.5 mg/1 IBA and IBA 0.5 mg/1 + LAA 0.5 mg/1. Eighty to ninety per cent regenerated plantlets were viable with 85% relative humidity while transferred to sterilized soil.

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References

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Published

27-05-2001

How to Cite

[1]
S. . Rahman, M. . Mamun, M. . Karim, and A. . Rahman, “IN VITRO REGENERATION OF PROMISING SUGARCANE VARIETIES OF BANGLADESH”, Khulna Univ. Stud., pp. 465–469, May 2001.

Issue

Section

Life Science

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