THE EFFECT OF PLANT GROWTH REGULATORS ON CALLUS  INDUCTION AND REGENERATION OF HAPLOID PLANTLETS  IN ANTHER CULTURE OF WHEAT

Md. Monirul  Islam; G. I.  Ivanov; Mahtalat  Ahmed

doi:10.53808/KUS.1999.1.2.273-276-Ls

Authors

Md. Monirul Islam Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
G. I. Ivanov Department of Biotechnology, Kuban State Agrarian University, Krasnodar, Russia.
Mahtalat Ahmed Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.1999.1.2.273-276-Ls

Keywords:

Wheat; Anther culture; Callus induction; Regeneration; Growth regulators

Abstract

The experiment was conducted to assess the effect of different concentration combination of 2,4D, a-NAA and kinetin on callus induction and regeneration of haploid in anther culture of wheat. Potato-2 liquid medium supplemented with different concentration combinations was used for this purpose. Among all the formulations, efficient callus induction and plant regeneration were observed in liquid potato medium containing following concentrations (mg l^-1) of plant growth regulators: 2,4D-0.75; a-NAA-0.1 and kinetin 0.75. This formulation supported to produce haploid plantlets directly without transferring calli to regeneration medium.

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