ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF BACTERIAL PATHOGEN IN COMMERCALLY IMPORTANT SHRIMP AND FISH COLLECTED FROM GHERS IN SATKHIRA DISTRICT, BANGLADESH
DOI:
https://doi.org/10.53808/KUS.SI.FRCC.2006.123-127-lsKeywords:
Bacterial content, opportunistic pathogen, Vibrio, Penaeus monodon, Lates calcariferAbstract
Investigations were carried out for isolation, enumeration and characterization of bacterial pathogens from shrimp Penaeus monodon and vetki Lates calcarifer from different ghers at Asasuni thana of Satkhira district. Shrimp and vetki were randomly sampled with a cast net from each gher individually during the period of September to December, 2005. After sampling, the live specimens of 20 shrimp and 12 fish were brought to the laboratory and immediately dissected. Muscle, hepatopancreas and gill were removed aseptically, weighed and homogenized with 0.85% NaCl solution. The homogenate was diluted using consecutive decimal dilution and inoculated on standard agar plates for enumeration of total bacterial load. A total load of 106-7 CFU g-1, 105-6 CFU g-1 and 107-10 CFU/g-1 were found in hepatopancreas, muscle and gill of shrimp and 105-6 CFU g-1, 106-9 CFU g-1and 107-10 CFU ml-1 in fish muscle, gill and slime, respectively. The total bacterial load in water of the ghers was estimated to be 107-10 CFU ml-1. A total of 32 and 20 representative isolates were selected from shrimp and fish respectively to identify them up to the genus through morphological, physiological and biochemical properties. Four genera namely, Vibrio, Micrococcus, Aeromonas and Pseudomonas were identified from the samples. Vibrio (56%) was predominantly isolated followed by Micrococcus (19%), Aeromonas (16%) and Pseudomonas (9%) in P. monodon whereas Pseudomonas (45%) dominated in L. calcarifer followed by Aeromonas (30%), Micrococcus (15%) and Vibrio (10%). High bacterial load in shrimp, fish and water of the ghers, it can be assumed that shrimp culture of Satkhira district is susceptible to bacterial contamination specially vibrio which is one of the devastating pathogens causing vibriosis in our country.
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