EFFECT OF SALT STRESS ON CHLOROPHYLL AND PROLINE  ACCUMULATION IN LEAVES OF Heritiera fomes Buch.-Ham. AND  Xylocarpus moluccensis (Lam.) M. Roem. SEEDLINGS REGULATING  ITS SURVIVAL AND GROWTH

S.M. Rubaiot Abdullah; Mahmood Hossain; Basubi Binti  Zhilik; Tanjit Rubaiat; Mohammad Raqibul Hasan Siddique; Sanjoy   Saha

doi:10.53808/KUS.2019.16.1and2.1814-L

EFFECT OF SALT STRESS ON CHLOROPHYLL AND PROLINE ACCUMULATION IN LEAVES OF Heritiera fomes Buch.-Ham. AND Xylocarpus moluccensis (Lam.) M. Roem. SEEDLINGS REGULATING ITS SURVIVAL AND GROWTH

Authors

S.M. Rubaiot Abdullah Forestry and Wood Technology Discipline, Khulna University, Khulna 9208, Bangladesh
Mahmood Hossain Forestry and Wood Technology Discipline, Khulna University, Khulna 9208, Bangladesh
Basubi Binti Zhilik Forestry and Wood Technology Discipline, Khulna University, Khulna 9208, Bangladesh
Tanjit Rubaiat Forestry and Wood Technology Discipline, Khulna University, Khulna 9208, Bangladesh
Mohammad Raqibul Hasan Siddique Forestry and Wood Technology Discipline, Khulna University, Khulna 9208, Bangladesh
Sanjoy Saha Center for Integrated Studies on Sundarbans (CISS), Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2019.16.1and2.1814-L

Keywords:

Chlorophyll, proline, salinity, Heritiera fomes, Xylocarpus moluccensis

Abstract

Heritiera fomes Buch.-Ham. and Xylocarpus moluccensis (Lam.) M. Roem. are the two most important trees of the Sundarbans. Survival and biomass increment, chlorophyll and proline concentration in leaves were investigated in seedlings of the two species grown at different levels of salinity in hydroponic culture. Seedlings were grown at 0, 5, 10, 15, 20, 25, 30 and 35 ppt salinities for 90 days. Significant reduction of chlorophyll concentration was found with increasing salinity for both the species. Total chlorophyll concentration halved for both the species as the salinity was increased from 0 to 35 ppt (0.34 to 0.17 mg/g for H. fomes and 0.07 to 0.03 mg/g for X. moluccensis). In addition, the H. fomes leaves have five times more chlorophyll concentration than X. moluccensis. Proline accumulation in leaves of X. moluccensis was found considerably high compared to H. fomes and increase with salinity for both the species. It was quadrapoled (254 to 1462 µmoles/g) in X. moluccensis and doubled (8 to 16 µmoles/g) in H. fomes. Seedling survival was found to vary with species and salinity levels. Survival of H. fomes seedlings decreased from 83% to 58% at 15 to 35 ppt salinity and no mortality was observed at lower salinities of 0 to 10 ppt. In contrast no mortality was observed from 0 to 25 ppt salinity in X. moluccensis seedlings and 92% seedling survived at 30 and 35 ppt salinities. Both the species showed lower biomass accumulation at higher salinities. Higher biomass increment (38 to 52%) was observed for X. moluccensis than that of H. fomes(33%) at lower salinities (0 to 5 ppt) and reduced gradually at higher salinities. This study revealed that X. moluccensis is more salt tolerant than H. fomes considering higher survival as a result of high proline accumulation in leaves that helps to overcome salt induced negative osmotic pressure. But growth is reduced due to lowering of chlorophyll accumulation at higher salinities for both the species.

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References

Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts, polyphenol oxidase in Beta vulgaris. Plant Physiology, 24(1): 1-15. https://doi.org/10.1104/pp.24.1.1

Aziz, I., & Khan, M. A. (2001). Experimental assessment of salinity tolerance of Ceriops tagal seedlings and saplings from the Indus delta, Pakistan. Aquatic Botany, 70(3): 259-268. https://doi.org/10.1016/S0304-3770(01)00160-7

Ball, M.C. (2002). Interactive effects of salinity and irradiance on growth: implications for mangrove forest structure along salinity gradients. Trees, 16(2-3): 126-139. https://doi.org/10.1007/s00468-002-0169-3

Bates, L.S., Waldren, R. P., & Teara, I. D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39(1): 205-207. https://doi.org/10.1007/BF00018060

Cardona-Olarte, P., Twilley, R. R., Krauss, K. W., & Rivera-Monroy, V. (2006). Responses of neotropical mangrove seedlings grown in monoculture and mixed culture under treatments of hydroperiod and salinity. Hydrobiologia, 569(1): 325-341. https://doi.org/10.1007/s10750-006-0140-1

Falqueto, A. R., Silva, D. M., & Fontes, R. V. (2008). Photosynthetic performance of mangroves Rhizophora mangle and Laguncularia racemosa under field conditions. Revista Árvore, 32(3): 577-582. http://dx.doi.org/10.1590/S0100-67622008000300018

Flowers, T. J., & Colmer, T. D. (2008). Salinity tolerance in halophytes. New Phytologist, 179(4): 945-963. DOI:10.1111/j.1469-8137.2008.02531.x

Hiscox, J. D., & Israelstam, G. E. (1979). A method for extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany, 57(12): 1332-1334. https://doi.org/10.1139/b79-163

Hogarth, P.J. (2015) The biology of mangroves and seagrasses. Third edition, Oxford university press.

Hoque, A.K.F., Hossain, I., Limon, S.H., & Khairuzzaman, M. (2006). Effect of salinity on germination and seedling growth of Aegiceras corniculatum (L.) Blanco. Khulna University studies. Special Issue (1st 15 Research Cell Conference): 141-146

Hussain, Z., & Karim, A. (1994). Introduction. In Z. Hussain, & G. Acharya (eds.), Mangrove of the Sundarbans, vol 2: Bangladesh. IUCN, Bangkok, Thailand (pp. 1-10)

Iftekhar, M. S., & Saenger, P. (2008). Vegetation dynamics in the Bangladesh Sundarbans mangroves: a review of forest inventories. Wetland Ecology and Management, 16(4): 291-312. https://doi.org/10.1007/s11273-007-9063-5

Islam, S., Feroz, S.M., Ahmed, Z. U., Chowdhury, A.H., Khan, R.I., & Al-Mamun, A. (2016). Species richness and diversity of the floristic composition of the Sundarbans mangrove reserve forest, Bangladesh in relation to spatial habitats and salinity. The Malaysian Forester, 79 (1&2): 7-38

Lopez-Hoffman, L., Anten, N. P. R., Martinez-Ramos, M., & Ackerly, D. D. (2007). Salinity and light interactively affect neotropical mangrove seedlings at the leaf and whole plant levels. Oecologia, 150(4): 545-556. https://doi.org/10.1007/s00442-006-0563-4

Lopez-Hoffman, L., DeNoyer, J. L., Monroe, I., Shaftel, R., Anten, N. P. R., Martinez-Ramos, M., & Ackerly, D. D. (2006). Mangrove seedling net photosynthesis, growth, and survivorship are interactively affected by salinity and light. Biotropica, 38(5): 606-616. https://doi.org/10.1111/j.1744-7429.2006.00189.x

Mitra, A., & Banerjee, K. (2010). Pigments of Heritiera fomes seedlings under different salinity conditions: perspective sea level rise. Mesopotamian Journal of Marine Science, 25(1): 1-10

Munns, R. (1993). Physiological processes limiting plant growth in saline soils: some dogmas and hypotheses. Plant, Cell and Environment, 16(1): 15-24. https://doi.org/10.1111/j.1365-3040.1993.tb00840.x

Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59: 651-681. https://doi.org/10.1146/annurev.arplant.59.032607.092911

Nandy (Datta), P., Dasgupta, N., & Das, S. (2009). Differential expression of physiological and biochemical characters of some Indian mangroves towards salt tolerance. Physiology and Molecular Biology of Plants, 15(2): 151-160. doi: 10.1007/s12298-009-0017-7

Nasrin, S., Mahmood, H., Abdullah, S. M. R., Alam, M. R., Saha, S., & Siddique, M. R. H. (2016). Salinity influence on survival, growth and nutrient distribution in different parts of Millettia pinnata (L.) Panigrahi seedlings. Agriculture and Forestry, 62(4): 161-173. doi: 10.17707/AgricultForest.62.4.19

Omoto, E., Taniguchi, M., & Miyake, H. (2010). Effects of salinity stress on the structure of bundle sheath and mesophyll chloroplasts in NAD-malic enzyme and PCK type C4

Parida, A. K., & Das, A.B. (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60(3): 324-349.

Plant Production Science, 13(2): 169-176. doi: 10.1626/pps.13.169

Panda, D., Dash, P. K., Dhal, N. K., & Rout, N. C. (2006). Chlorophyll fluorescence parameters and chlorophyll content in mangrove species grown in different salinity. General and Applied Plant Physiology, 32(3-4): 175-180.

Parida, A.K., Das, A. B., & Das, P. (2002). NaCl stress causes changes in photosynthetic pigments, proteins and other metabolic components in the leaves of a true mangrove, Bruguiera parviflora, in hydroponic cultures. Journal of Plant Biology, 45(1): 28-36. https://doi.org/10.1007/BF03030429

Parida, A.K., Das, A.B., Sanada, Y., & Mohanty, P. (2004). Effects of salinity on biochemical components of the mangrove, Aegiceras corniculatum. Aquatic Botany, 80(2): 77-87. https://doi.org/10.1016/j.aquabot.2004.07.005

https://doi.org/10.1016/j.ecoenv.2004.06.010>

Popp, M., & Albert, R. (1995). The role of organic solutes in salinity adaptations of mangroves and herbaceous halophytes. In M. A. Khan & I. A. Ungar (eds.), Biology of Salt Tolerant Plants. Department of Botany, University of Karachi (pp. 139-149)

Popp M., Larher F., & Weigel P. (1985) Osmotic adaption in Australian mangroves. In W.G.

Beeftink, J. Rozema & A.H.L. Huiskes (eds.), Ecology of coastal vegetation. Advances in vegetation science, vol 6. Springer, Dordrecht (pp. 247-253)

Qiu, D. L., Lin, P., & Guo, S. Z. (2007). Effects of salinity on leaf characteristics and CO2/H2O exchange of Kandelia candel (L.) Druce seedlings. Journal of Forest Science, 53(1): 13-19. https://doi.org/10.17221/2081-JFS

Rajesh, A., Arumugam, R., & Venkatesalu, V. (1999). Responses of Ceriops roxburghiana to NaCl stress. Biologia Plantarum42: 143-148. https://doi.org/10.1023/A:1002189425061

Saenger, P. (2002). Mangrove ecology, silviculture and conservation. Kluwer Academic Publishers, Dordrecht.

Sarker, S. K., Reeve, R., Thompson, J., Paul, N. K., & Matthiopoulos, J. (2016). Are we failing to protect threatened mangroves in the Sundarbans world heritage ecosystem? Scientific Reports, 6, 21234. DOI: 10.1038/srep21234

Siddiqi, N. A. (2001). Mangrove forestry in Bangladesh. Institute of Forestry and Environmental Sciences, University of Chittagong, Chittagong, Bangladesh

Takemura, T., Hanagata, N., Sugihara, K., Baba, S., Karube, I., & Dubinsky, Z. (2000). Physiological and biochemical responses to salt stress in the mangrove, Bruguiera gymnorrhiza. Aquatic Botany, 68: 15-28. https://doi.org/10.1016/S0304-3770(00)00106-6

Tomlinson, P. B. (1986). The Botany of Mangroves. Cambridge University Press, Cambridge.

Wu, Q. S., & Zou, N. Y. (2009). Adaptive responses of birch-leaved pear (Pyrus betulaefolia) seedlings to salinity stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37: 133-138.

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Published

25-12-2019

How to Cite

[1]

S. R. Abdullah, M. Hossain, B. B. Zhilik, T. Rubaiat, M. R. H. Siddique, and S. . Saha, “EFFECT OF SALT STRESS ON CHLOROPHYLL AND PROLINE ACCUMULATION IN LEAVES OF Heritiera fomes Buch.-Ham. AND Xylocarpus moluccensis (Lam.) M. Roem. SEEDLINGS REGULATING ITS SURVIVAL AND GROWTH”, Khulna Univ. Stud., pp. 9–17, Dec. 2019.