MODULATORY ANTI-DIARRHEAL EFFECTS OF ASCORBIC ACID IN SWISS ALBINO MICE

Mahmuda Akter  Mukta; Rajib  Hossain; Farhana  Faria; Md Shahajul  Islam; Chandan  Sarkar; Abul Bashar Ripon  Khalipha; Olubunmi  Atolani; Muhammad Torequl  Islam

doi:10.53808/KUS.2021.18.01.2103-L

Authors

Mahmuda Akter Mukta Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj (Dhaka)-8100, Bangladesh.
Rajib Hossain Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj (Dhaka)-8100, Bangladesh.
Farhana Faria Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj (Dhaka)-8100, Bangladesh.
Md Shahajul Islam Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj (Dhaka)-8100, Bangladesh.
Chandan Sarkar Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj (Dhaka)-8100, Bangladesh.
Abul Bashar Ripon Khalipha Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj (Dhaka)-8100, Bangladesh.
Olubunmi Atolani Department of Chemistry, University of Ilorin, PMB 1515, Ilorin, Nigeria
Muhammad Torequl Islam Department of Pharmacy, Life Science Faculty, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj (Dhaka)-8100, Bangladesh.

DOI:

https://doi.org/10.53808/KUS.2021.18.01.2103-L

Keywords:

Ascorbic acid; Castor oil; Diarrhea; Mus musculus; Prazosin

Abstract

Ascorbic acid (AA) has been reported for the management of diarrhea. The anti-diarrheal potential and modulatory activities of AA on some commonly used anti-diarrheal drugs were investigated. For this purpose, the activities of AA on castor oil-induced diarrhea in Swiss mice were examined. As standard anti-diarrheal agents, we used prazosin, propranolol, loperamide, and nifedipine with or without AA. The results revealed that AA at 25 mg/kg (i.p.) and all other standard drugs exhibited significant (p < 0.05) diarrheal attenuating activities in mice. However, the impact was more pronounced in the loperamide and propranolol groups. AA administrated with prazosin and propranolol had a higher rate of latent periods and a lower rate of diarrheic secretion during the study period (4 h) than that of the other single or mixed groups. Furthermore, a molecular docking study illustrated that AA displayed good binding affinities with (α1) (–5.2 Kcal/mol), α2b (–5.4 Kcal/mol), α2c (-5.6 Kcal/mol), β1(–5.3 Kcal/mol) and β2(–6.4 Kcal/mol) adrenoceptors. Of note, AA exerted a significant anti-diarrheal effect and it was seen to modulate the anti-diarrheal effects of α- and β-adrenergic receptor blocking agents in Swiss mice.

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