EFFECT OF SUBCULTURING AND CULTURE AGE ON  SPORULATION AND MORPHOLOGICAL CHARACTERISTICS OF  Bipolaris sorokinian

Zilkad  Nasrin; Zannat Ara  Ferdousy; Bidhan Chandro  Sarker; Sabiha  Sultana; Sanjoy Kumar  Adhikary; Chhoa  Mondal

doi:10.53808/KUS.2017.14.1and2.1627-L

Authors

Zilkad Nasrin Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
Zannat Ara Ferdousy Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
Bidhan Chandro Sarker Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
Sabiha Sultana Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
Sanjoy Kumar Adhikary Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
Chhoa Mondal Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2017.14.1and2.1627-L

Keywords:

Subculture, culture age, sporulation, Bipolaris sorokiniana

Abstract

Two in vitro experiments were conducted to evaluate the effect of successive sub-culturing on sporulation, morphological characteristics and culture age for the production of conidia of three isolates of Bipolaris sorokiniana of which two were from wheat (BS-1 and BS-2) and one from barley (BS-3). The experiments were conducted following standard procedure. After every successive subculturing, number of spores gradually declined in all three isolates. In first two isolates spores were produced up to fourth subculture, but no spore was observed at fifth subculture. In isolate BS-3 the spores were produced up to second subculture and no spore was found at third subculture. There was significant variation among these three isolates for the production of spores in each subculture. Variation was found in margin, shape, color, texture, zonation and arial mycelium in top view and reverse view after 12 days of inoculation. Variation was also observed in conidia and conidiophore of the three isolates at 12 days age culture. In isolates BS-1, BS-2 and BS-3 the highest conidia production were obtained in 14 days (10620 x103 conidia/ml), 18 days (578 x103 conidia/ml) and 10 days (7.8 x103 conidia/ml) old culture, respectively. In the isolates BS-1 and BS-2, number of conidia was increased up to 14 days and 18 days, respectively and then decreased. In isolate BS-3 the highest number of conidia was found at 10 days old culture but decreased gradually with the age.

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