Antibiotic Sensitivity of Vibrio spp. Isolated from the Hatchery Water of Tiger Shrimp Penaeus monodon
DOI:
https://doi.org/10.53808/KUS.2026.SI.7.01.MariNEX1505-lsKeywords:
Shrimp Hatchery, Bacterial diseases, Susceptibility, AMRAbstract
Penaeus monodon, one of the most traded aquatic species, fetch a premium price in national and international markets for its significant taste and nutritional value. But, vibriosis caused by Vibrio spp. is a threat to the sustainability of shrimp aquaculture. This study aimed at identifying and profiling antibiotic resistance of Vibrio spp. detected in the water samples, collected from June to September of 2023 from four shrimp hatcheries located in Cox's Bazar, Batiaghata and Dacope Upazillas of Khulna District and Debhata Upazilla of Satkhira Ditrict. The colonies of Vibrio spp. from TCBS isolation, included Vibrio alginolyticus (9.55±0.14×105 CFU /ml) from Cox’s Bazar samples, Vibrio fluvialis (9.55±0.21×106 CFU /ml) from Dacope samples, and Vibrio furnissii (3.66±0.41×106 CFU/ml) and Vibrio parahaemolyticus (3.55±0.23×105 CFU/ml) from Debhata samples in accordance with biochemical tests. Considering two replications of all the experiments, the antibiotic sensitivity test (Kirby-Bauer method) determined that all Vibrio isolates were resistant to penicillin, 75% to erythromycin, 50% to ampicillin, 25% to oxytetracycline, nalidixic acid and azithromycin. The V. alginolyticus and V. furnissii isolates had multidrug resistance (MDR) with Multiple Antibiotic Resistance (MAR) values of 0.41 and 0.17, respectively. In contrast, all the tested isolates were susceptible to cefotaxime, chloramphenicol, sulphamethoxazole & trimethoprim, nitrofurantoin, ciprofloxacin, and tobramycin. Therefore, it has been evident that the shrimp hatchery's water is very prone to antibiotic resistant Vibrio spp. contamination with 5-41% MDR. This study recommends an effective implementation of biosecurity measures to reduce the transmission of disease from other sources and the driving factors associated with vibriosis in P. monodon hatchery must be identified.
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