A COMPARATIVE STUDY ON BRAIN-TROPISM OF BETANODAVIRUSES IN YOUNG MARINE FISH: A WAY TO PERFORM HOST SPECIFICITY

Authors

  • G. R. Banu Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna 9208, Bangladesh
  • K. Mori Kamiura Station, Japan Sea-Farming Association, Oita 879-2602, Japan
  • T. Nakai Fish Pathobiology Laboratory, Graduate School of Biosphere Science, Hiroshima University, Higashihiroshima 738-8528, Japan

DOI:

https://doi.org/10.53808/KUS.2006.7.1.0602-L

Keywords:

Betanodavirus, marine fish, host-specificity, intavitreous injection, brain-tropism

Abstract

Investigation on the brain-tropism was carried out through direct injection of the virus into the vitreous body of the eye using five marine fish species: kelp grouper, striped jack, Japanese flounder, threeline grunt and yellow tail. When the brains of intravitreously-injected fish (106 TCID50 Fish-1) were examined at 72 h post infection, striped jack nervous necrosis virus (SJNNV; the type species of the genus Betanodavirus) was frequently detected from striped jack and flounder while kelp grouper nervous necrosis virus (KGNNV) were detected from kelp grouper, striped jack, and flounder. No constant growth of the virus was noticed in the brains of threeline grunt and yellow tail. Considering that natural outbreaks of VNN (viral nervous necrosis) have been frequently reported in the former three species but not in the later two. The present result supports that the brain cell-tropism might be one of the tools to perform the host-specificity of betanodavirus.

Downloads

Download data is not yet available.

References

Breuil, G.; Bonami, J.R.; Pepin, J.F. and Pichot, Y. 1991. Viral infection (picorna-like virus) associated with mass mortalities with hatchery reared sea-bass (Dicentrarchus labrax) larvae and juveniles. Aquaculture, 97: 109-116.

Frerichs, G.N.; Rodger, H.D. and Peric, Z. 1996. Cell culture isolation of piscine neuropathy nodavirus from juvenile sea bass, Dicentrarchus labrax. Journal of General Virology, 77: 2067-2071.

Ikenaga, T.; Tatecho, Y.; Nakai, T. and Uemastu, K. 2002. Betanodavirus as nobel transneural tracer for fish. Neuroscience Letter, 331: 55-59.

Iwamoto, T.; Nakai, T.; Mori, K.; Arimoto, M. and Furusawa, I. 2000. Cloning of fish cell line SSN-1 for piscine nodaviruses. Diseases of Aquatic Organisms, 43, 81-89.

Munday, B.L.; Kwang, J. and Moody, N. 2002. Betanodavirus infections of teleost fish: a review. Journal of Fish Diseases, 25: 127-142.

Munday, B.L.; Langdon, J.S.; Hyatte, A. and Humphrey, J.D. 1992. Mass mortality associated with a viral-induced vacuolating encephalopathy and retinopathy of larval and juvenile barramundi, Lates calcarifer Bloch. Aquaculture, 103: 197-211.

Nguyen, H.D.; Nakai, T. and Muroga, K. 1996. Progression of striped jack nervous necrosis virus (SJNNV) infection in naturally and experimentally infected striped jack Pseudocaranx dentex larvae. Diseases of Aquatic Organisms, 24: 99-105.

Nishizawa, T.; Furuhashi, M.; Nagai, T.; Nakai, T. and Muroga, K. 1997. Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene. Applied and Environmental Microbiology, 63: 1633-1636.

Nishizawa, T.; Mori, K.; Furuhashi, M.; Nakai, T.; Furusawa, I. and Muroga, K. 1995. Comparison of the coat protein genes of five fish nodaviruses, the causative agents of viral nervous necrosis in marine fish. Journal of General Virology, 76: 1563-1569.

Nishizawa, T.; Mori, K.; Nakai, T.; Furusawa, I. and Muroga, K. 1994. Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis virus (SJNNV). Diseases of Aquatic Organisms, 18:103-107.

Peducasse, S.; Castric, J.; Thiery, R.; Jeffroy, J.; Van, A.L. and Baudin, F.L. 1999. Comparative study of viral encephalopathy and retinophathy in juvenile sea bass Dicentrarchus labrax infected in different ways. Diseases of Aquatic Organisms, 36: 11-20.

Reed, L.J. and Muench, H. 1938. A simple method of estimating fifty percent endpoints. American Journal of Hygiene, 27: 493-497.

Tanaka, S.; Aoki, H. and Nakai, T. 1998. Pathogenecity of the nodavirus detected from diseased sevenband grouper Epinephelus septemfasciatus. Fish Pathology, 33: 31-36.

Totland, G.K.; Grotmol, S.; Morita, Y.; Nishioka, T. and Nakai, T. 1999. Pathogenecity of nodavirus strains from striped jack Pseudocaranx dentex and Atlantic halibut Hippoglossus hippoglossus, studied by waterborne challenge of yolk-sac larvae of both teleost species. Diseases of Aquatic Organisms, 38: 169-175.

Yoshikoshi, K. and Inoue, K. 1990. Viral nervous necrosis in hatchery-reared larvae and juveniles of Japanese parrotfish, Oplegnathus fasciatus (Temminck and Schlegel). Journal of Fish Diseases, 13: 69-77.

Downloads

Published

26-05-2006

How to Cite

[1]
G. R. . Banu, K. . Mori, and T. . Nakai, “A COMPARATIVE STUDY ON BRAIN-TROPISM OF BETANODAVIRUSES IN YOUNG MARINE FISH: A WAY TO PERFORM HOST SPECIFICITY”, Khulna Univ. Stud., pp. 45–48, May 2006.

Issue

Section

Life Science

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.