A COMPARATIVE STUDY ON BRAIN-TROPISM OF BETANODAVIRUSES IN YOUNG MARINE FISH: A WAY TO PERFORM HOST SPECIFICITY

G. R.  Banu; K.  Mori; T.  Nakai

doi:10.53808/KUS.2006.7.1.0602-L

Authors

G. R. Banu Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna 9208, Bangladesh
K. Mori Kamiura Station, Japan Sea-Farming Association, Oita 879-2602, Japan
T. Nakai Fish Pathobiology Laboratory, Graduate School of Biosphere Science, Hiroshima University, Higashihiroshima 738-8528, Japan

DOI:

https://doi.org/10.53808/KUS.2006.7.1.0602-L

Keywords:

Betanodavirus, marine fish, host-specificity, intavitreous injection, brain-tropism

Abstract

Investigation on the brain-tropism was carried out through direct injection of the virus into the vitreous body of the eye using five marine fish species: kelp grouper, striped jack, Japanese flounder, threeline grunt and yellow tail. When the brains of intravitreously-injected fish (10⁶TCID₅₀Fish^-1) were examined at 72 h post infection, striped jack nervous necrosis virus (SJNNV; the type species of the genus Betanodavirus) was frequently detected from striped jack and flounder while kelp grouper nervous necrosis virus (KGNNV) were detected from kelp grouper, striped jack, and flounder. No constant growth of the virus was noticed in the brains of threeline grunt and yellow tail. Considering that natural outbreaks of VNN (viral nervous necrosis) have been frequently reported in the former three species but not in the later two. The present result supports that the brain cell-tropism might be one of the tools to perform the host-specificity of betanodavirus.

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