PRODUCTION POTENTIAL OF ACETIC AND LACTIC ACID OF PROBIOTIC Bifidobacterium iactis

S. M. E.  Babar; Sudip K.  Rakshit

doi:10.53808/KUS.2002.4.1.0155-L

Authors

S. M. E. Babar Biotechnology Discipline, Khulna University, Khulna-9208. Bangladsh.
Sudip K. Rakshit Bioprocess Technology, Asian Institute of Technology, Klongluang, Pathumthanil2l20, Thailand

DOI:

https://doi.org/10.53808/KUS.2002.4.1.0155-L

Keywords:

Bifidobacteria, probiotic, prebiotic, fructooligosaccharides.

Abstract

Bifidobacterium Iactis was grown in laboratory to observe the acetic and lactic acid production by the cells. Cells were grown in the laboratory in MRS-IM media where carbohydrate sources were varied. This media was defined by the Christian Hansen, Denmark, who provided the freeze-dried cell sample. A one-liter glass fermenter was used to grow the organisms. Three different carbohydrates source viz. 2% (w/v) glucose, 2% (w/v) glucose + 2% (v/v) soymilk, and 2% (w/v) fructooligosaccharides (FOS) were used. After preparing the product, samples were analyzed in Shimadzu gas chromatograph, where acetic and lactic acid concentration was measured.

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References

Babar, S. M. E. and Rakshit, S. K., (2001).Prebiotics selection and Optimization of Cultural Condition for probiotic Bifidobacterium lactis, Khulna university Studies, Khulna , Bangladesh, 3(2):557-564.

Conway, P.L, 1995. Microbial Ecology of the Human Large Intestine. In Human Colonic Bacteria: Role in Nutrition. Physiology, and Pathology, eds. G. R. Gibson and G. T. Macfarlane, CRC Press, Boca Raton, Florida, pp.: 1-24.

Drasar, B.S., 1990. Bifidobacterium. Topley & Wilson's principles of Bacteriology. Virology and Immunity: Systematic Bacteriology, eds. M. T. Parker and L. H. Collier, B. C. Decker Inc.,Philadelphia, 2:580-583.

Gibson, G.R., Cummings, J.H., and Macfarlane, G.T., 1991. Growth and Activities of Sulfate-Reducing Bacteria in Gut contents of Healthy Subjects and Patients with Ulcerative Colitis. FEMS Microbiology Ecology, 77: 412-420.

Gibson, G.R., and Macfarlane, G.T., 1994. Intestinal Bacteria and Disease. Human health: Contribution of Microorganisms, ed., S.A.W. Gibson, Spinger-Verlag, London, pp.: 53-62.

Gibson, G.R., and Roberfroid, M.B., 1995. Dietary Modulation of the Human Colonic Microbiota: Introducing the Concept of Prebiotics. Journal of Nutrition, 125: 1401-1412.

Gibson, G.R., Willems, A., Reading, S., and Collins, M.D., 1996. Fermentation of Non-Digestible Oligosaccharides by Human Colonic Bacteria. Proceedings of the Nutrition Society, 55: 899-912.

Mckellar R.C., and Molder H.W., 1989. Metabolism of Fructo-oligosaccharides by Bifidobacterium spp. Applied Microbiology and Biotechnology, 31: 537-541.

Miele, L., Ludwig, U., Gut, C., Kaufmann, P., Dasen, G., Wegner, S., and Teuber, M., 1997.Bifidobacterium lactiss sp. Nov., A moderatcely Oxygen Tolerant Species Isolated from Fermented Milk. Systematic Applied Microbiology, 20: 20-57.

Mitsuoka T., 1984. Taxonomy and Ecology of Bifidobacteria. Bifidobacteria Microflora, 3: 11-28.

Mitsuoka T., 1982. Recent Trades in Research on Intestinal Flora. Bifidobacteria Microflora, 1: 3-24.

Scardovi V, 1986. Genus Bifidobacterium. Bergey's Manual of Systematic bacteriology. Eds. N.R. Craig and L.C. Holt, Williams and Wilkins, New York, 2: 1418-1434.

Ziemer C.J., and Gibson G.R. 1998. An Overview of Probiotics, Prebiotics and Synbiotics in the Functional Food Concept: Perspectives and Future Strategies. International Dairy Journal, 8: 473-479.