PREBIOTICS SELECTION AND OPTIMIZATION OF CULTURAL CONDITION FOR PROBIOTIC BIFIDOBACTERIUM LACTIS

S.M.E.  Babar; S.K.  Rakshit

doi:10.53808/KUS.2001.3.2.0154-L

Authors

S.M.E. Babar Biotechnology Discipline, Khulna University, Bangladesh.
S.K. Rakshit Bioprocess Technology, Asian Institute of Technology, Bangkok, Thailand.

DOI:

https://doi.org/10.53808/KUS.2001.3.2.0154-L

Keywords:

Bifidobacterium lactis, probiotic, fructooligosaccharides

Abstract

Bifidobacterium lactis, a well-known probiotic, was cultured in laboratory to observe the effect of substrate and mixing on cell growth. The cells were grown in MRS-IM media defined by Christian Hansen, Denmark, where carbohydrate sources were varied. Glucose, fructooligosaccharides (FOS), soy broth and commercial soymilk were used as carbohydrate sources. A one-liter bioreactor was used to grow the cells, where anaerobic condition was maintained by supplying nitrogen gas throughout the experiment. Fructooligosaccharides, and commercial soymilk were found effective on this cell growth. The overall cell growth was found to be almost indifferent, but growth rate was considerably higher due to mixing.

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References

Fuller, R. and Gibson, G.R., 1997. Modification of the Intestinal Microflora Using Probiotics and Prebiotics. Gastroenterology,32, Suppl. 222: 28-31.

Gibson, G.R., Cummings, J.H. and Macfarlane, G.T., 1991. Growth and Activities of Sulfate-Reducing Bacteria in Gut contents of Healthy Subjects and Patients with Ulcerative Colitis. FEMS Microbiology Ecology,77: 412-420.

Gibson, G.R. and Macfarlane, G.T., 1994. Intestinal Bacteria and Disease. Human health: Contribution of Microorganisms, ed., S.A.W. Gibson, Spinger-Verlag, London, pp.: 53-62.

Gibson, G.R. and Roberfroid, M.B., 1995. Dietary Modulation of the Human Colonic Microbiota: Introducing the Concept of Prebiotics. Journal of Nutrition, 125: 1401-1412.

Gibson, G.R., Willems, A., Reading, S. and Collins, M.D., 1996. Fermentation of Non-Digestible Oligosaccharides by Human Colonic Bacteria. Proceedings of the Nutrition Society,55: 899-912.

Gibson, G.R., Berry Ottaway, P. and Rastall, R.A. (2000) Prebiotics: New Developments in Functional Foods.Chandos Publishing Limited, Oxford.

Gibson, G.R., Prebiotics for gut health, International Food Information Service, <http://www.ifis.org/index.html>. seen on 2 July, 2001.

Hidaka H., Eida T., Takizawa T., Tokunagga T. and Tashiro Y., 1986. Effects of Fructooligosaccharides on Intestinal Flora and Human Health. Bifidobacteria Microflora,5: 37-50.

Klahorst, S J., 2000. Contributing Editor, Pro-and Prebiotics for health, Functional Foods Annual, USA <http://www.foodproductdesign.com/archive/20Q0/0900ffa O6.html#top>. Seen on 3 July 2001.

Lee Y.K. and Salminen S., 1995. The Coming Age of Probiotics. Trend in Food science and Technology, 6: 241-245.

Macfarlane G.T. and Gibson G. R., 1994. Metabolic Activities of the Normal Colonic Flora. Human Health: The contribution of Microorganisms, pp. 17-52.

Mckellar R.C. and Molder H.W., 1989. Metabolism of Fructo-oligosaccharides by Bifidobacterium spp. Applied Microbiology and Biotechnology,31:537-541.

Miele, L. Ludwig, U., Gut, C., Kaufmann, P., Dasen, G., Wegner, S., and Teuber, M., 1997. Bifidobacterium lactiss sp. Nov., A moderateely Oxygen Tolerant Species Isolated from Fermented Milk. Systematic Applied Microbiology, 20: 20-57.

Mitsuoka T., 1984. Taxonomy and Ecology of Bifidobacteria. Bifidobacteria Microflora,3: 11-28.

Mitsuoka T„Hidaka H. and Eida T., 1987. Effects of Fructooligosaccharides on Intestinal Microflora. Die Nahrung, 31: 427-436.

O'Sullivan M.G., 1996. Metabolism of Bifidogenic Factors by Gut Flora- an Overview. Bulletin of the IDF 313: Oligosaccharides and Probiotic Bacteria, 23-30.

Roberfroid M.,B, 1996. Functional Effects of Food Components and the Gastrointestinal System: Chicory Fructooligosaccharides. Nutrition Reviews,54:S38-S42.

Rowland I.R., 1995. Toxicology of the Colon- Role of the Intestinal Microflora. Human Colonic Bacteria: Role in Nutrition, Physiology, and Pathology (eds.), G. R. Gibson and G. T. Macfarlane, CRC Press, Boca Raton, Florida, pp.: 155-174.

Salminen S., Bousley C., Boutron-Ruault M.-C. Cummings J.H., Gibson G.R., Isolauri E. Moreau M.-C., Roberfroid M. and Rowland L., 1998. Functional Food Science and Gastrointestinal Physiology and Function. Brit. Journal of Nutrition, Suppl. 1: 5147-5171.

Scardovi V, 1986. Genus Bifidobacterium. Bergey's Manual of Systematic bacteriology. Eds. N.R. Craig and L.C. Holt, Williams and Wilkins, New York, 2: 1418-1434.

Sonomoto, K„ Etoh, S.-I., Oiki, H. and Ishizaki, A., 1998. Growth-Stimulation of Bifidobacterium by Natural Rubber Serum Powder. Annals New York Academy of Sciences, 864; 502-505.

Sultana K., God3ward G., Reynolds N., Arumugaswamy R„ Peiris P. and Kailasapathy K, 2000. Encapsulation of Probiotic Bacteria with Alginate-Starch and Evaluation of Survival in Simulated

Gastrointestinal Conditions and in Yoghurt. International Journal of Food Microbiology, 62; 47-55.

Tannock G.W., 1995a. Normal Microflora: an Introduction to Microbes Inhabiting the Human Body, Chapman and Hall, London.

Tannock G.W., 1995b. Role of Probiotics. Human Colonic Bacteria: Role in Nutrition, Physiology, and

Pathology,eds. G. R. Gibson and G. T. Macfarlane, CRC Press, Boca Raton, Florida, pp.: 257-271.

Willis A.T., 1991. Anaerobic Culture Methods. Anaerobic Microbiology: A Practical Approach, ed. P.N. Levett, IRL Press, Oxford University Press, UK, pp. 1-11.

Ziemer C.J., and Gibson G.R., 1998. An Overview of Probiotics, Prebiotics and Synbiotics in the Functional Food Concept: Perspectives and Future Strategies. International Dairy Journal, 8: 473-479.