EFFECT OF SEASON AND GROWTH REGULATORS ON IN VITRO PROPAGATION OF JACKFRUIT (ARTOCARPUS HETEROPHYLLUS LAM)

M. A.  Mannan; Habiba  Nasrin; M. M.  Islam

doi:10.53808/KUS.2006.7.2.0617-L

Authors

M. A. Mannan Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh
Habiba Nasrin Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh
M. M. Islam Agrotechnology Discipline, Khulna University, Khulna-9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2006.7.2.0617-L

Keywords:

Growth regulators, In Vitro propagation, Jackfruit

Abstract

The present study was conducted at the Laboratory of Plant Tissue Culture of Agrotechnology Discipline, Khulna University, Khulna, during the period from September, 2004 to May, 2005 with a view to study the effect of time of the year and growth regulators on in vitro propagation of jackfruit. Shoot tips from fresh sprouts on the trunk of mature jackfruit trees (Artocarpus heterophyllus Lam.) were used as explants. The explants were collected at three different times of the year from the same jackfruit plants viz. 5/09/04, 18/10/04, 5/01/05. The collected explants were cultured on MS medium supplemented with NAA-0.5 mgl^-1 and fortified with different levels (1.0, 1.5 and 2.0 mgl^-1) of BA to study the proliferation and multiplication of shoots from the cultured shoot tips and their subsequent growth and development. The in vitro shoot bud proliferation, multiplication and survivability were found better when the explants were cultured in January. BA at a concentration of 1.5 mgl^-1 was found most suitable for shoot proliferation and multiplication. And all the three concentrations of BA varied significantly. The survivability of the proliferated shoot buds was found better in concentration of BA at 1.0 and 1.5 mgl^-1.

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References

Amin, M.N. and Jaiswal, V.S. 1993. In vitro response of apical bud explants from mature trees of jackfruit (Artocarpus heterophyllus Lam.). Plant Cell Tissue Organ Culture, 33: 59-65.

Anon. 2000. Yearbook of Agricultural Statistics of Bangladesh. Bangladesh Bureau of Statistics, Ministry of Planning, Government of the People’s Republic of Bangladesh.

Dhar, M. 1998. Techniques of vegetative and in vitro propagation of Jackfruit. Ph.D. Thesis, Banggabandhu Shaikh Mujibar Rahman Agricultural University, Salna, Gazipur, Bangladesh, 120 pp.

Gomez, K.A. and Gomez, A.A. 1984. Statistical Procedures for Agricultural Research. 2nd edn., International Rice Research Institute, Manila, Philippines, pp. 188-198.

Hammerschlag, F.A. 1986. Temperate Fruits and Nuts. In: Zimmerman, R.H.; Griesbach, R.J.; Hammerschlag, F.A. and Lawson, R.H. (eds.), Tissue Culture as a Plant Production System for Horticultural Crops. Tissue Culture as a Plant Production System for Horticultural Crops, Murtinus Nijhoff Publication, Dordrecht, pp. 221-236.

Mott, R.L. 1981. Trees. In: Conger, B.V. (ed.), Cloning Agricultural Plants via in vitro Techniques. CRC Press, Boca Ratan, pp. 217-254.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiology of Plant, 15: 473- 497.

Prokash, J. 1993. Large-scale production of tissue culture plantlets of economic crops. In: Srivastava, H.C. (ed.), Biotechnological Applications for Food Security in Developing Countries. Oxford & IBH Pub. Co. Pvt. Ltd., New Delhi, pp. 273-274.

Roy, S.K.; Rahman, S.K.L. and Majumder, R. 1990. Propagation of a timber tree, Artocarpus heterophyllus through in vitro culture. Journal of Horticultural Science, 65: 355-358.

Samaddar, H.N. 1990. Jackfruit. In: Bose, T.K. and Mitra, S.K. (eds.), Fruits: Tropical and Sub-tropical. Naya Prokash, Calcutta, pp. 638-649.

Singh, A. 1986. Fruit Physiology and Production. Kalyani Publishers, Calcutta, 512 pp.