CALLUS INDUCTION AND PLANT REGENERATION IN ANTHER CULTURE OF RICE (Oryza sativa L.)

M. M .  Islam; S.M.K.  Shaifullah; M. G.  Raihan

doi:10.53808/KUS.2002.4.1.0111-L

Authors

M. M . Islam Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
S.M.K. Shaifullah Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
M. G. Raihan Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2002.4.1.0111-L

Keywords:

Rice, Anther, Callus, Plant regeneration, Haploid.

Abstract

The effect of genotypes and physical state (liquid and semisolid) of the N6 medium on callus formation and subsequent plant regeneration from cultured anthers of six rice varieties (Oryza sativa L.) were examined. Significant differences were observed among the genotypes in regard to anther response to callus induction and subsequent plant regeneration. Average callus induction rate in liquid and in semisolid media did not differ significantly. Callus induction frequency of the genotypes varied from 1.2% to 64.0% and regeneration frequency varied from 0 to 56.5%. The traditional variety Chini Atab produced both callus and regenerated plants in maximum frequencies; 64% and 56.5% respectively.

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