THE EFFECT OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND REGENERATION OF HAPLOID PLANTLETS IN ANTHER CULTURE OF WHEAT

Authors

  • Md. Monirul Islam Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh
  • G. I. Ivanov Department of Biotechnology, Kuban State Agrarian University, Krasnodar, Russia.
  • Mahtalat Ahmed Agrotechnology Discipline, Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.1999.1.2.273-276-Ls

Keywords:

Wheat; Anther culture; Callus induction; Regeneration; Growth regulators

Abstract

The experiment was conducted to assess the effect of different concentration combination of 2,4D, a-NAA and kinetin on callus induction and regeneration of haploid in anther culture of wheat. Potato-2 liquid medium supplemented with different concentration combinations was used for this purpose. Among all the formulations, efficient callus induction and plant regeneration were observed in liquid potato medium containing following concentrations (mg l-1) of plant growth regulators: 2,4D-0.75; a-NAA-0.1 and kinetin 0.75. This formulation supported to produce haploid plantlets directly without transferring calli to regeneration medium.

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References

Bakumovsky, G.V.U., 1983. Plant Growing in the Tropics and Subtropics. Mir Publishers, Moscow, pp. 96.

Butenko, R.G., Kholodov and Urmantsev, B.B., 1972. Growth pattern and some corelations between growth and content of sugar in the plant cell. Plant Physiology, 19: 926 – 936.

George, H.L., 1987. Direct generation of wheat haploids via anther culture. Crop Science, 27: 336-339.

Henry, Y., De Buyser, J., 1984. Wheat microspore embryogenesis during in vitro anther culture. Theoritical and Applied Genetics, 67: 439-442.

Huang, B., 1985. Effect of plant growth subtanaces on callus formation and plant regeneration in anther culture of rice. IRRI Notes, Los Banos, The Philippines, pp. 149-164.

Ouyang, J.W., Hu H., Chuang C.C. and Tseng, C.C., 1973. Induction of haploid plants from anthers of Tr. aestivum cultured in vitro. Science Sinica, 16: 79-95.

Zu, Z.O., 1978. Cytological investigation on androgenesis of Tr. aestivum. Acta Botany Sinica, 20: 6-12.

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Published

26-11-1999

How to Cite

[1]
M. M. . Islam, G. I. . Ivanov, and M. . Ahmed, “THE EFFECT OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND REGENERATION OF HAPLOID PLANTLETS IN ANTHER CULTURE OF WHEAT”, Khulna Univ. Stud., pp. 273–276, Nov. 1999.

Issue

Section

Life Science

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