IN VITRO CLONAL PROPAGATION OF ONION (ALLIUM CEPA L.) THROUGH SHOOT TIP CULTURE

Authors

  • Shefali Boonerjee Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh
  • Palash Kumar Sen Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, Bangladesh
  • Md. Firoz Alam Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh
  • S.M. Mahbubur Rahman Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2006.7.1.0409-L

Keywords:

Onion, cytokinin, auxin, shoot tip, plantlet

Abstract

The aim of the present investigation was to standardize the in vitro shoot tip culture technique considering various culture aspects for direct plant formation of two onion (Allium cepa L.) varieties ‘Indian’ and ‘Taherpuri’. About 1.5-2.2 mm shoot tips were collected from bulbs and were sterilized with 0.1% HgCl2 in various duration. Then they were cultured on MS medium supplemented with various hormonal concentrations and different media composition for primary establishment. BAP 1.5 mg l-1 + NAA1.0 mg l-1 was found to be the best formulation for primary establishment of shoot tips. Primarily established shoot tips were further cultured for shoot multiplication, and 2ip 2.0 mg l-1 + NAA 0.5 mg l-1 was found the most effective for shoot multiplication. The highest percentage of shootlet produced root on MS+2.0 mg l-1 IBA for both the cultivars (Taherpuri 90% and Indian 80%). The plantlets were transferred to small pots containing soil : sand 1:1 and kept under shade and covered with perforated polythene sheet. After proper hardening the plantlets were transferred to field.

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References

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Published

26-05-2006

How to Cite

[1]
S. . Boonerjee, P. K. . Sen, M. F. . Alam, and S. M. . Rahman, “IN VITRO CLONAL PROPAGATION OF ONION (ALLIUM CEPA L.) THROUGH SHOOT TIP CULTURE”, Khulna Univ. Stud., pp. 49–53, May 2006.

Issue

Section

Life Science

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