MICROPROPAGATION OF ONION (ALLIUM CEPA L.)  THROUGH BULBLET FORMATION

Shefali  Boonerjee; Palash Kumar  Sen; Md. Firoz  Alam; S.M. Mahbubur  Rahman

doi:10.53808/KUS.2006.7.1.0501-L

Authors

Shefali Boonerjee Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh
Palash Kumar Sen Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, Bangladesh
Md. Firoz Alam Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, Bangladesh
S.M. Mahbubur Rahman Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2006.7.1.0501-L

Keywords:

In vitro, Onion, Bulblet, Cytokinin, Auxin

Abstract

Multiple shoot clumps or microclones were collected from two onion (Allium cepa L.) varieties for bulblet formation. MS medium supplemented with various concentrations of BAP, Kn, 2ip and NAA were used. The height responded shoot was observed 90% and 80% in Taherpuri and Indian variety, respectively on MS supplemented with 2.0 mg l^-1 BAP+0.5 mg l^-1 NAA. The best bulblet formation was obtained in MS containing 1.0 mg l^-1 2ip. Finally, the rooted bulblets were hardened and established in soil under natural environment.

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