STUDY ON IDENTIFICATION OF PLOIDY LEVEL OF REGENERATED PLANTS OBTAINED FROM CULTURED WHEAT ANTHERS USING   LENGTH OF GUARD CELLS

Md. Monirul  Islam; Mahtalat  Ahmed; G.I.  Ivanov; P.  Gain

doi:10.53808/KUS.2000.2.1.119-122-Ls

Authors

Md. Monirul Islam Discipline of Agrotechnology, Khulna University, Khulna 9208, Bangladesh
Mahtalat Ahmed Discipline of Agrotechnology, Khulna University, Khulna 9208, Bangladesh
G.I. Ivanov Department of Biotechnology, Kuban State Agrarian University, Krasnodar, Russia
P. Gain Discipline of Agrotechnology, Khulna University, Khulna 9208, Bangladesh

DOI:

https://doi.org/10.53808/KUS.2000.2.1.119-122-Ls

Keywords:

Guard cell; Ploidy level; Diploid; Haploid; Chromosome number

Abstract

A fast and reliable method for ploidy determination of regenerated Triticum aestivum from anther culture is described in the present work. The anthers of 47 hybrid combinations of winter wheat were cultured in vitro and the length of the guard cells was measured in regenerated plants. Thus, plants with a guard cell length of 32-46 mm were haploid, whilst plants with a guard cell length of 54-74 mm were diploid. Plants with a guard cell of 46-54 mm required further chromosome investigation for the exact determination of ploidy level.

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References

Chase, S.S., 1964. Monoploids and diploids of maize: a comparison of genotypic equivalents. American Journal of Botany, 51: 924-933.

Chuang, C.C., Ouyang, T.W., Chia, H., Chou, S.M. and Ching, C.K., 1978. A set of potato media for wheat anther culture. Proceedings of Symposium on Plant Tissue Culture, Science Press, Peking, 51-56.

Daophen, X., Chunkhon, L. and Dhanpen, L., 1991. Length of the guard cells as indicator of ploidy of wheat plants, obtained through anther culture. TAA Bulletin, Tashkent, 3: 53-57.

Du-LP., Xu, H.J., Zhao, L.L., Zhang, C.X. and Ren, X., 1996. Identification of the ploidy of wheat pollen plants using the leangth of guard cells. Ningxia Journal of Agriculture and Forestry Science and Technology, 5: 10-12.

Karpechenko, P.O., 1935. Experimental methods for obtaining polyploids and haploids. In: Fundamentals of Plant Breeding. Moscow, pp. 197-434.

Murashige, T. and S koog, F., 1962. A revised medium for rapid growth and bioassay tobacco tissue culture, Plant Physiology, 15: 473-497.

Waninge, J., 1965. A modified method of counting chromosomes in root tip cells of wheat. Euphytica, 14: 249-250.

Zaitsev, M.E., 1968. Dete rmination of hoploid plants in maize by using anatomical elements. In: Apomixis and Cytoembryology of Plants. Saratov, pp. 142-149.